Programmable and selective silencing of oncogenic fusion transcripts by CRISPR-Cas13b via breakpoint sequence recognition.
Hu 胡文欣 W, Chen 陈竑霖 H, He S, Casan JML, Shembrey C, Li S, Brown LM, Zhao A, Truong L, Buchner I, Vassiliadis D, Hughes TP, White DL, Carmichael CL, De Weck A, Voskoboinik I, Gouil Q, Trapani JA, Ekert PG, Sadras T, Fareh M
Sci Adv. 2026 Oct 16;12(42):eady0296. doi: 10.1126/sciadv.ady0296. Epub 2026 Oct 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 262237 | MSCV-BCR-ABL1-p190 (T315I)-IRES-EGFP | Retroviral expression plasmid used to generate cells that stably express the BCR::ABL1 p190 isoform carrying the T315I kinase-domain mutation. |
| 262238 | MSCV-BCR(fragment)-ABL1-p190-3xHA-IRES-mCherry | Retroviral expression plasmid used to express the BCR-ABL1 breakpoint fragment |
| 262239 | MSCV-ABL1(fragment)-3xHA-IRES-eGFP | Retroviral expression plasmid used to express the ABL1 fragment |
| 262240 | MSCV-BCR (fragment)-3xHA-IRES-tagBFP | Retroviral expression plasmid used to express the BCR fragment |
| 262241 | MSCV-NPM-ALK-IRES-mCherry | Retroviral expression plasmid used to express NPM-ALK |
| 262242 | MSCV-SS18-SSX (fragment)-IRES-mcherry | Retroviral expression plasmid used to express SS18-SSX fragment |
| 262243 | MSCV-EWSR1 WT (fragment)-IRES-EGFP | Retroviral expression plasmid used to express EWS-WT exons 7-11 |
| 262244 | MSCV-FLI1 WT (fragment)-IRES-EGFP | Retroviral expression plasmid used to express FL1-WT exons 4-9 |
| 262245 | MSCV-EWSR1-FLI1 type1 (fragment)-IRES-EGFP | MSCV-EWSR1-FLI1 -Type1 = Exon 6/7 breakpoint, cloned using ~500bp fragment of cDNA from A673 cell line |
| 262246 | MSCV-EWSR1-FLI1 type2 (fragment)-IRES-EGFP | MSCV-EWSR1-FLI1-Type2 = Exon 7/5 breakpoint, cloned from the ES1 breakpoint |