Cre-lox-regulated conditional RNA interference from transgenes.
Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T
Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 11578 | pSico | Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression. |
| 11579 | pSicoR | Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression. |
| 11586 | pSico PGK puro | Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression. |
| 12084 | pSicoR PGK puro | Cre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression. |
| 12089 | pSico p53 | Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating p53 shRNA expression. |
| 12090 | pSicoR p53 | Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF p53 shRNA expression. |
| 12093 | pSico PGK GFP | Cre-regulated lentiviral shRNA vector. Cre addition causes the EGFP gene to be recombined out of the construct, activating shRNA expression. |
| 12165 | pSico Dnmt1 | Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating Dnmt1 shRNA expression. |
| 12166 | pSicoR Dnmt1 | Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF Dnmt1 shRNA expression. |