pSicoR
(Plasmid #11579)

• Purpose: (Empty Backbone) Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.
• Depositing Lab: Tyler Jacks
• Depositing Organization: Massachusetts Institute of Technology
  Located in United States of America
• Publication: Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
• Citations: 73 Articles

Backbone

• Vector backbone: pSicoR
• Backbone size (bp): 7567
• Modifications to backbone: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: mU6-F (5'-ATATCCCTTGGAGAAAAGCCTT-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSicoR allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map for pSico and on the Jacks Lab website http://web.mit.edu/jacks-lab/protocols_table.html