Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional activator complex.
Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, Zhang H, Takahashi JS
Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804. Epub 2012 May 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47334 | pCMV10/3xflag-Clock | full length clock in pCMV10 with 3x flag at the N-terminus |
| 47335 | pfastbac/mBmal62-447 | mBmal fragment cloned into pfastbac and crystallized |
| 47336 | pfastbacHTb/mClock26-384 | mClock fragment cloned into pfastbacHTb vector with Hexa His tag |
| 47337 | pCMV10/3xflag-Clock L57E | Mutation used for co-IPs |
| 47338 | pCMV10/3xflag-Clock L74E | Mutation used for co-IPs |
| 47339 | pCMV10/3xflag-Clock L113E | Mutation used for co-IPs |
| 47340 | pCMV10/3xflag-Clock F122D | Mutation used for co-IPs |
| 47341 | pCMV10/3xflag-Clock W284A | Mutation used for co-IPs |
| 47342 | pCMV10/3xflag-Clock V315R | Mutation used for co-IPs |
| 47343 | pCMV10/3xflag-Clock L57E/L113E/W284A | triple mutation used for co-IP |
| 47344 | pCDNA3-HA/Bmal L95E | PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003 |
| 47345 | pCDNA3-HA/Bmal L115E | PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003 |
| 47346 | pCDNA3-HA/Bmal L150E | PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003 |
| 47347 | pCDNA3-HA/Bmal I317D | PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003 |
| 47348 | pCDNA3-HA/BmalF423R | PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003 |
| 47349 | pCDNA3-HA/Bmal W427A | PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003 |
| 47350 | pCDNA3-HA/Bmal V435R | PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003 |
| 47351 | pCDNA3-HA/Bmal F423R/V435R/W427R | PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003 |
| 47352 | pCDNA3-HA/Bmal L95E/L150E/W427A | PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003 |
| 47353 | HLH Clock Pas 1-401 VenN | Clock fragment cloned and tagged with VenN |
| 47354 | HLH Clock Pas 1-401 VenN L57E | Clock fragment mutatation tagged with VenN |
| 47355 | HLH Clock Pas 1-401 VenN L74E | Clock fragment mutatation tagged with VenN used for BiFC |
| 47356 | HLH Clock Pas 1-401 VenN L113E | Clock fragment mutatation tagged with VenN used for BiFC |
| 47357 | HLH Clock Pas 1-401 VenN F122D | Clock fragment mutatation tagged with VenN used for BiFC |
| 47358 | HLH Clock Pas 1-401 VenN W284A | Clock fragment mutatation tagged with VenN used for BiFC |
| 47359 | HLH Clock Pas 1-401 VenN V315R | Clock fragment mutatation tagged with VenN used for BiFC |
| 47360 | HLH Clock Pas 1-401 VenN L57E/L113E/W284A | Clock fragment mutatation tagged with VenN used for BiFC |
| 47364 | HLH Bmal Pas 1-465-VenC | Bmal fragment cloned with Venus tag and used for BiFC |
| 47365 | HLH Bmal Pas 1-465-VenC L95E | Bmal fragment cloned with Venus tag used for mutation |
| 47366 | HLH Bmal Pas 1-465-VenC L150E | Bmal fragment cloned with Venus tag used for mutation |
| 47367 | HLH Bmal Pas 1-465-VenC I317D | Bmal fragment cloned with Venus tag used for mutation |
| 47368 | HLH Bmal Pas 1-465-VenC F423R | Bmal fragment cloned with Venus tag used for mutation |
| 47369 | HLH Bmal Pas 1-465-VenC W427A | Bmal fragment cloned with Venus tag used for mutation |
| 47370 | HLH Bmal Pas 1-465-VenC V435R | Bmal fragment cloned with Venus tag used for mutation |
| 47371 | HLH Bmal Pas 1-465-VenC F423R/V435R | Bmal fragment cloned with Venus tag used for mutation |
| 47372 | HLH Bmal Pas 1-465-VenC L95E/L150E/W427A | Bmal fragment cloned with Venus tag used for mutation |
| 47374 | LV-CMV-Cer-FL- Bmal L95E | Bmal full length with cer tag used for mutation |
| 47376 | LV-H2B-mRFP1 | This was used as a nuclear marker |
| 47377 | HLH Bmal Pas 1-465-VenC L115E | Bmal fragment cloned with Venus tag used for mutation |