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A prodrug resistance mechanism is involved in colibactin biosynthesis and cytotoxicity.
Brotherton CA, Balskus EP
J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. Epub 2013 Feb 20. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
48243pET29b-ClbPpep-CHisFirst 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain
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48244pET29b-ClbPUntagged, full-length protein for in vivo peptidase assays
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48245pET29b-ClbP-S95AUntagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays
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49217pet28a-ClbB-NRPS-NHisNRPS module of ClbB containing CAT domains with N-terminal 6His tag
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51497pET28a-ClbN-NHisNRPS module containing CATE domains with N-terminal 6His tag
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Recombinant Antibodies from Article