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Competing E3 ubiquitin ligases govern circadian periodicity by degradation of CRY in nucleus and cytoplasm.
Yoo SH, Mohawk JA, Siepka SM, Shan Y, Huh SK, Hong HK, Kornblum I, Kumar V, Koike N, Xu M, Nussbaum J, Liu X, Chen Z, Chen ZJ, Green CB, Takahashi JS
Cell. 2013 Feb 28;152(5):1091-105. doi: 10.1016/j.cell.2013.01.055.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
48752pCMV10-Flag-Fbxl21Expression of FLAG-tagged Fbxl21
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51888pCMV10-Flag-Ovtmexpression vectore in mammalian cells
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51890pTre2tet)::Fbxl21expression of Fbxl21
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51892pfast bac cry1mycBackbone plasmid is pfastBacHTa from invitrogen
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51893pfast bac Rbx-HABackbone plasmid is pfastBacHTa from invitrogen
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51894pfast bac-flag Fbxl3Backbone plasmid is pfastBacHTa from invitrogen
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110297mcry1K31R HA tagmammalian expression of full length mouse CRY1 mutant K31R
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110298mCRY1 HA tagfull length wt mouse Cry1 HA tag in pcDNA3.1
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Antibodies from Article