Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas.
Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW
Genetics. 2013 Aug 26. PubMed Article
Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW
Genetics. 2013 Aug 26. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47911 | SP6-hCas9-Ce-mRNA | In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase. |
| 47912 | SP6-sgRNA-scaffold | Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection |