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Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas.
Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW
Genetics. 2013 Aug 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
47911SP6-hCas9-Ce-mRNAIn vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
47912SP6-sgRNA-scaffoldScaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

Antibodies from Article