Purpose(Empty Backbone) Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47912||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3000
Vector typeWorm Expression, CRISPR
- Promoter SP6
/ Fusion Protein
- sgRNA 3' end (C terminal on insert)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byContains sequence adapted from Addgene plasmid #41824 "gRNA Empty Vector"
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SP6-sgRNA-scaffold was a gift from Paul Sternberg (Addgene plasmid # 47912 ; http://n2t.net/addgene:47912 ; RRID:Addgene_47912)
For your References section:Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas. Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW. Genetics. 2013 Aug 26. 10.1534/genetics.113.155879 PubMed 23979577