Functional Expression of Human NKCC1 from a Synthetic Cassette-Based cDNA: Introduction of Extracellular Epitope Tags and Removal of Cysteines.
Somasekharan S, Monette MY, Forbush B
PLoS One. 2013 Dec 5;8(12):e82060. doi: 10.1371/journal.pone.0082060. PubMed
Somasekharan S, Monette MY, Forbush B
PLoS One. 2013 Dec 5;8(12):e82060. doi: 10.1371/journal.pone.0082060. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 49060 | pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13) | Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence |
| 49061 | pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51) | Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49063 | pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931) | Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49064 | pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933) | Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49065 | pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935) | Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49066 | pcDNA3.1 Flag YFP hNKCC1 C295S (NT103) | Expresses human NKCC1 C295S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49067 | pcDNA3.1 Flag YFP hNKCC1 C295A (NT104) | Expresses human NKCC1 C295A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49068 | pcDNA3.1 Flag YFP hNKCC1 C543M (NT360) | Expresses human NKCC1 C543M mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49069 | pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864) | Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49070 | pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865) | Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49071 | pcDNA3.1 Flag YFP hNKCC1 C630A (NT399) | Expresses human NKCC1 C630A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49072 | pcDNA3.1 Flag YFP hNKCC1 C630S (NT400) | Expresses human NKCC1 C630S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49073 | pcDNA3.1 Flag YFP hNKCC1 C723S,C724V (NT501) | Expresses human NKCC1 C723S and C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49074 | pcDNA3.1 Flag YFP hNKCC1 C791S (NT572) | Expresses human NKCC1 C791S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49075 | pcDNA3.1 Flag YFP hNKCC1 C820S (NT573) | Expresses human NKCC1 C820S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49076 | pcDNA3.1 Flag YFP hNKCC1 C1052S (NT674) | Expresses human NKCC1 C1052S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49077 | pcDNA3.1 HA CFP hNKCC1 WT (NT15-H) | Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence |
| 49078 | pcDNA3.1 Flag YFP hNKCC1 TM-cysteineless (NT859) | Expresses human NKCC1 mutant lacking cysteine residues within transmembrane domains and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic,containing convenient restriction sites |
| 49079 | pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868) | Expresses human NKCC1 mutant lacking cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49080 | pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855) | Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 49085 | pcDNA3.1 Flag YFP hNKCC1 (NT17) | Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence |