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pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)
(Plasmid #49077)


Item Catalog # Description Quantity Price (USD)
Plasmid 49077 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pcDNA3.1 hygro
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 5484
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    human NKCC1 (synthetic)
  • Alt name
    Slc12A2 (synthetic)
  • Alt name
    Na-K-Cl cotransporter
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
  • Mutation
    262 silent mutations from native hNKCC1
  • Entrez Gene
    SLC12A2 (a.k.a. BSC, BSC-2, BSC2, CCC1, KILQS, NKCC1, PPP1R141)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3x HA (N terminal on insert)
    • mCerulean (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Synthetic cDNA encoding full length human NKCC1, with 29 silent restriction sites in NKCC1 coding region

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 HA CFP hNKCC1 WT (NT15-H) was a gift from Biff Forbush (Addgene plasmid # 49077 ; ; RRID:Addgene_49077)
  • For your References section:

    Functional Expression of Human NKCC1 from a Synthetic Cassette-Based cDNA: Introduction of Extracellular Epitope Tags and Removal of Cysteines. Somasekharan S, Monette MY, Forbush B. PLoS One. 2013 Dec 5;8(12):e82060. doi: 10.1371/journal.pone.0082060. PubMed 24339991