The RNA polymerase dictates ORF1 requirement and timing of LINE and SINE retrotransposition.
Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM
PLoS Genet. 2009 Apr;5(4):e1000458. doi: 10.1371/journal.pgen.1000458. Epub 2009 Apr 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51283 | pAluYa5-neo-TET | expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette |
| 51284 | L1-neo-TET | expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette |
| 51285 | p7SL-neo-TET | expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette |
| 51286 | pU6-neo-TET | expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette |
| 51287 | pB2-neo-TET | expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette |