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A promiscuous lipid-binding protein diversifies the subcellular sites of toll-like receptor signal transduction.
Bonham KS, Orzalli MH, Hayashi K, Wolf AI, Glanemann C, Weninger W, Iwasaki A, Knipe DM, Kagan JC
Cell. 2014 Feb 13;156(4):705-16. doi: 10.1016/j.cell.2014.01.019.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
52736PLC-TIR-GFPRetroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker
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52737PX-TIR-GFPRetroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker
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52738SLP2a-TIR-GFPRetroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker
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52739TIRAP-GFPRetroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker
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52740TIRAP-TAP_IRES_GFPRetroviral vector expresses hTIRAP-3xHA and a target site for the biotin ligase BirA upstream of IRES and GFP
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Antibodies from Article