PX-TIR-GFP
(Plasmid #52737)

• Purpose: Retroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker
• Depositing Lab: Jonathan Kagan
• Publication: Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10.1016/j.cell.2014.01.019.

Backbone

• Vector backbone: MSCV2.2
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: human CD2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Toll/IL-1 Receptor adaptor protein
• Alt name: TIRAP
• Alt name: Mal
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• Mutation: Deleted amino acids 1-85
• GenBank ID: AF378129.1 TIRAP
• Entrez Gene: TIRAP (a.k.a. BACTS1, Mal, MyD88-2, wyatt)
• Tags / Fusion Proteins:
  • p40 Phox lipid binding domain (N terminal on insert)
  • GFP (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry