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Analysis of G protein betagamma dimer formation in live cells using multicolor bimolecular fluorescence complementation demonstrates preferences of beta1 for particular gamma subunits.
Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
55191YFP(159-238)-gamma-5 in pcDNAI/AmpA carboxyl-terminal YFP fragment was fused to Ggamma-5. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55192YFP(159-238)-gamma-10 in pcDNAI/AmpA carboxyl-terminal YFP fragment was fused to Ggamma-10. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55193YFP(159-238)-gamma-11 in pcDNAI/AmpA carboxyl-terminal YFP fragment was fused to Ggamma-11. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55194YFP(159-238)-gamma-12 in pcDNAI/AmpA carboxyl-terminal YFP fragment was fused to Ggamma-12. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55592CFP(159-238)-beta-1 in pcDNAI/AmpA carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
55615CFP(1-158)-gamma-1 in pcDNAI/AmpAn amino-terminal CFP fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55616CFP(1-158)-gamma-2 in pcDNAI/AmpAn amino-terminal CFP fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55617CFP(1-158)-gamma-5 in pcDNAI/AmpAn amino-terminal CFP fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55618CFP(1-158)-gamma-7 in pcDNAI/AmpAn amino-terminal CFP fragment was fused to Ggamma-7. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55619CFP(1-158)-gamma-10 in pcDNAI/AmpAn amino-terminal CFP fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55620CFP(1-158)-gamma-11 in pcDNAI/AmpAn amino-terminal CFP fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55621CFP(1-158)-gamma-12 in pcDNAI/AmpAn amino-terminal CFP fragment was fused to Ggamma-12. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55622mRFP-memThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.
55623Cer(1-158)-gamma-1 in pcDNAI/AmpAn amino-terminal mCerulean fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55624Cer(1-158)-gamma-2 in pcDNAI/AmpAn amino-terminal mCerulean fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55625Cer(1-158)-gamma-5 in pcDNAI/AmpAn amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55626Cer(1-158)-gamma-7 in pcDNAI/AmpAn amino-terminal mCerulean fragment was fused to Ggamma-7. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55627Cer(1-158)-gamma-10 in pcDNAI/AmpAn N-terminal mCerulean fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55628Cer(1-158)-gamma-11 in pcDNAI/AmpAn N-terminal mCerulean fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55629Cer(1-158)-gamma-12 in pcDNAI/AmpAn N-terminal mCerulean fragment was fused to Ggamma-12. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
60895CFP(1-158) in pcDNAI/AmpThis vector attaches CFP(1-158) to the N-terminus of a protein.
60896CFP(159-238) in pcDNAI/AmpThis vector attaches CFP(159-238) to the N-terminus of a protein.
60897Cerulean(1-158) in pcDNAI/AmpThis vector attaches Cerulean(1-158) to the N-terminus of a protein.

Antibodies from Article