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YFP(159-238)-gamma-10 in pcDNAI/Amp
(Plasmid #55192)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55192 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNAI/Amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 5233
  • Modifications to backbone
    A BglII site was introduced to into the polylinker of pcDNAI/Amp 3' to the BamHI site.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP-(159-238)-gamma-10
  • Alt name
    GNG10
  • Species
    H. sapiens (human); Aequorea victoria
  • Insert Size (bp)
    433
  • Mutation
    Ggamma-10 was amplified by PCR, which added a BamHI site and a linker sequence (Arg-Ser) to the 5' end and a 3' BglII site. Cloning into the BglII site of YFP(159-238) destroyed the BamHI site.
  • Entrez Gene
    GNG10
  • Promoter CMV
  • Tag / Fusion Protein
    • YFP(159-238) was fused to the amino terminus of Ggamma-10. (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pEYFP was purchased from Clontech. Gamma-10 cDNA was obtained from Janet Robishaw, Weis Center for Research, Geisinger Clinic, Danville, PA.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP(159-238)-gamma-10 in pcDNAI/Amp was a gift from Catherine Berlot (Addgene plasmid # 55192 ; http://n2t.net/addgene:55192 ; RRID:Addgene_55192)
  • For your References section:

    Analysis of G protein betagamma dimer formation in live cells using multicolor bimolecular fluorescence complementation demonstrates preferences of beta1 for particular gamma subunits. Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH. Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26. 10.1124/mol.106.022616 PubMed 16641313