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Dominant-negative synthesis suppression of voltage-gated calcium channel Cav2.2 induced by truncated constructs.
Raghib A, Bertaso F, Davies A, Page KM, Meir A, Bogdanov Y, Dolphin AC
J Neurosci. 2001 Nov 1;21(21):8495-504.
PubMed

Plasmids from Article

ID Plasmid Purpose
58737Rabbit GFP Cav 2.2 pMT2expression of GFP tagged rabbit Cav2.2 in mammalian cells
89891pMT2 Cav2.2 DomI and DomIIMammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2
206076Cav2.1 HA pCAGGSexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II
206094Cav2.2 DomI + DomII pcDNA3expression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel
206095GFP Cav2.2 DomI + DomII pMT2expression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel with a N-terminal GFP tag
206099EYFP Cav2.2 pRK5expression of rabbit Cav2.2 calcium channel with a N-terminal EYFP tag and an exofacial double HA tag in domain II
206127Cav2.2 DomI pMT2expression of the N-terminus, Domain I and the I-II loop (amino acids 1-483) of rabbit Cav2.2 calcium channel

Antibodies from Article