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The 50:50 method for PCR-based seamless genome editing in yeast.
Horecka J, Davis RW
Yeast. 2014 Mar;31(3):103-12. doi: 10.1002/yea.2992. Epub 2013 Dec 13. PubMed Article

Plasmids from Article

ID Plasmid Purpose
47554pJH136pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

Antibodies from Article