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pJH136
(Plasmid #47554)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47554 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPCR-Script Amp SK(+)
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 4121
  • Vector type
    PCR template
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    URA3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1160
  • Promoter Native URA3 promoter, -243 to -1.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SrfI (destroyed during cloning)
  • 3′ cloning site SrfI (destroyed during cloning)
  • 5′ sequencing primer GTTGTAAAACGACGGCCAGT
  • 3′ sequencing primer TCACACAGGAAACAGCTATGA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Primer sequences (5' to 3'): U2, CGTACGCTGCAGGTCGAC; D2, ATCGATGAATTCGAGCTCG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH136 was a gift from Ronald Davis (Addgene plasmid # 47554 ; http://n2t.net/addgene:47554 ; RRID:Addgene_47554)
  • For your References section:

    The 50:50 method for PCR-based seamless genome editing in yeast. Horecka J, Davis RW. Yeast. 2014 Mar;31(3):103-12. doi: 10.1002/yea.2992. Epub 2013 Dec 13. 10.1002/yea.2992 PubMed 24639370