IpO
(Plasmid #48233)

• Purpose: Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.
• Depositing Lab: Ronald Davis
• Publication: Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.1002/yea.3006. Epub 2014 Mar 20.

Backbone

• Vector backbone: pBluescript II SK (-)
• Backbone manufacturer: Agilent Technologies
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 4343
• Vector type: Routine cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: URA3 3' fragment
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 669
• Mutation: URA3 ORF from +216 to 80 bp downstream of the stop codon
• Promoter: none

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: EagI (not destroyed)
• 5′ sequencing primer: M13Rev

Gene/Insert 2

• Gene/Insert name: URA3 5' fragment
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 737
• Mutation: -242 upstream to URA3 ORF +495
• Promoter: URA3 promoter

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: M13For

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry