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Global analyses of the effect of different cellular contexts on microRNA targeting.

Nam JW, Rissland OS, Koppstein D, Abreu-Goodger C, Jan CH, Agarwal V, Yildirim MA, Rodriguez A, Bartel DP
Mol Cell. 2014 Mar 20;53(6):1031-43. doi: 10.1016/j.molcel.2014.02.013. Epub 2014 Mar 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
60788pIS1 ATP2B1Luciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and wild-type miR-155 sites
60789pIS1 mATP2B1Luciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and mutated miR-155 sites
60790pIS1 ELOVL6Luciferase reporter to measure miR-155 mediated differential repression. Contains ELOVL6 3' UTR and wild-type miR-155 sites
60791pIS1 mELOVL6Luciferase reporter to measure miR-155 mediated differential repression. Contains ELOVL6 3' UTR and mutated miR-155 sites
60792pIS1 EPAS1Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and wild-type miR-155 sites
60793pIS1 mEPAS1Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and mutated miR-155 sites
60794pIS1 HK1Luciferase reporter to measure miR-155 mediated differential repression. Contains HK1 3' UTR and wild-type miR-155 sites
60795pIS1 mHK1Luciferase reporter to measure miR-155 mediated differential repression. Contains HK1 3' UTR and mutated miR-155 sites
60796pIS1 IL6RLuciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and wild-type miR-155 sites
60797pIS1 mIL6RLuciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and mutated miR-155 sites
60798pIS1 INSIG2Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and wild-type miR-155 sites
60799pIS1 mINSIG2Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and mutated miR-155 sites
60800pIS1 LMBRD2Luciferase reporter to measure miR-155 mediated differential repression. Contains LMBRD2 3' UTR and wild-type miR-155 sites
60801pIS1 mLMBRD2Luciferase reporter to measure miR-155 mediated differential repression. Contains LMBRD2 3' UTR and mutated miR-155 sites
60802pIS1 LPIN1Luciferase reporter to measure miR-155 mediated differential repression. Contains LPIN1 3' UTR and wild-type miR-155 sites
60803pIS1 mLPIN1Luciferase reporter to measure miR-155 mediated differential repression. Contains LPIN1 3' UTR and mutated miR-155 sites
60804pIS1 PELOLuciferase reporter to measure miR-155 mediated differential repression. Contains PELO 3' UTR and wild-type miR-155 sites
60805pIS1 mPELOLuciferase reporter to measure miR-155 mediated differential repression. Contains PELO 3' UTR and mutated miR-155 sites

Antibodies from Article