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Michael J. Fox Foundation Plasmid Resource


MJFF_newlogo_2.jpg

Addgene is working with The Michael J. Fox Foundation (MJFF) along with individual laboratories to assemble a Parkinson's disease plasmid resource for the scientific research community.

MJFF has created a number of plasmids for expression of LRRK2 protein domain variants. Browse the full list below.

ID Plasmid Gene/Insert Vector Type Mutations
38005pET-21a(+)/MBP-His-PINK1PINK1 (Homo sapiens)Bacterial Expression
38006pET-21a(+)/MBP-HisMBP (Maltose binding protein) (Synthetic)Bacterial Expression
38008pFastBac1/MBP-HisMBP (Maltose binding protein) (Synthetic)Insect Expression ; Baculovirus expression
39529pENTR221 LRRK2 wtLRRK2 (Homo sapiens)Gateway compatible cloning vector
40364pBACgus4x/cdc37/LRRK2 1867-2186LRRK2 (Homo sapiens)Baculovirus WT aa1867-2186
40365pBACgus4x/cdc37/LRRK2 1867-2186_G2019SLRRK2 (Homo sapiens)Baculovirus aa1867-2186, G2019S
40366pBACgus4x/cdc37/LRRK2 1867-2186_C2024S/C2025SLRRK2 (Homo sapiens)Baculovirus aa1867-2186, C2024S/C2025S
40367pBACgus4x/cdc37/LRRK2 1867-2186_T2031ELRRK2 (Homo sapiens)Baculovirus aa1867-2186, T2031E
40368pBACgus4x/cdc37/LRRK2 1867-2141LRRK2 (Homo sapiens)Baculovirus WT, aa1867-2141
40369pBACgus4x/cdc37/LRRK2 1867-2161LRRK2 (Homo sapiens)Baculovirus aa1867-2161
40370pBACgus4x/cdc37/LRRK2 1867-2176LRRK2 (Homo sapiens)Baculovirus WT aa1867-2176
40371pBACgus4x/cdc37/LRRK2 1867-2161_G2019SLRRK2 (Homo sapiens)Baculovirus aa1867-2161, G2019S
40372pBACgus4x/cdc37/LRRK2 1867-2161_C2024S/C2025SLRRK2 (Homo sapiens)Baculovirus aa1867-2161, C2024S/C2025S
40373pBACgus4x/cdc37/LRRK2 1867-2161_T2031ELRRK2 (Homo sapiens)Baculovirus aa1867-2161, T2031E
40374pBACgus/LRRK2 1867-2186LRRK2 (Homo sapiens)Baculovirus WT aa1867-2186
40375pBACgus/LRRK2 C-HisLRRK2 (Homo sapiens)Baculovirus WT
40376pBACgus/LRRK2 1867-2141 C-HisLRRK2 (Homo sapiens)Baculovirus WT aa1867-2141
40377pBACgus/LRRK2 1867-2161 C-HisLRRK2 (Homo sapiens)Baculovirus WT aa1867-2161
40378pBACgus/LRRK2 1867-2176 C-HisLRRK2 (Homo sapiens)Baculovirus WT aa1867-2176
40379pBACgus/LRRK2 C-His_T2031ELRRK2 (Homo sapiens)Baculovirus aa1867-2186, T2031E
40380pBACgus/LRRK2 1867-2161 C-His_T2031ELRRK2 (Homo sapiens)Baculovirus aa1867-2161, T2031E
40381pBACgus/LRRK2 C-His_G2019SLRRK2 (Homo sapiens)Baculovirus aa1867-2186, G2019S
40382pBACgus/LRRK2 1867-2161 C-His_G2019SLRRK2 (Homo sapiens)Baculovirus aa1867-2161, G2019S
40383pBACgus/LRRK2 C-His_C2024S/C2025SLRRK2 (Homo sapiens)Baculovirus aa1867-2186, C2024S/C2025S
40384pBACgus/LRRK2 1867-2161 C-His_C2024S/C2025SLRRK2 (Homo sapiens)Baculovirus aa1867-2161, C2024S/C2025S
40385pBACgus4x/ParkinParkin (Homo sapiens)Baculovirus WT
40386pBACgus_Sumostar/LRRK2 1867-2186SUMO (Synthetic), LRRK2 (Homo sapiens)Baculovirus WT aa1867-2186
40387pBACgus_Sumostar/LRRK2 1867-2186_G2019SSUMO (Synthetic), LRRK2 (Homo sapiens)Baculovirus aa1867-2186, G2019S
40388pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025SSUMO (Synthetic), LRRK2 (Homo sapiens)Baculovirus aa1867-2186, C2024S/C2025S
40389pBACgus_Sumostar/LRRK2 1867-2186_T2031ESUMO (Synthetic), LRRK2 (Homo sapiens)Baculovirus aa1867-2186, T2031E
40390pBACgus_Sumostar/LRRK2 1867-2141SUMO (Synthetic), LRRK2 (Homo sapiens)Baculovirus aa1867-2141
40391pBACgus_Sumostar/LRRK2 1867-2161SUMO (Synthetic), LRRK2 (Homo sapiens)Baculovirus aa1867-2161
40392pBACgus_Sumostar/LRRK2 1867-2176SUMO (Synthetic), LRRK2 (Homo sapiens)Baculovirus aa1867-2176
40393pBACgus_Sumostar/LRRK2 1867-2161_G2019SSUMO (Synthetic), LRRK2 (Homo sapiens)Baculovirus aa1867-2161, G2019S
40394pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025SSUMO (Synthetic), LRRK2 (Homo sapiens)Baculovirus aa1867-2161,C2024S/C2025S
40395pBACgus_Sumostar/LRRK2 1867-2161_T2031ESUMO (Synthetic), LRRK2 (Homo sapiens)Baculovirus aa1867-2161, T2031E
40396pBACgus4x/cdc37/RocCOR LRRK2 1867-2141LRRK2 (Homo sapiens)Baculovirus WT aa1867-2141
40397pBACgus4x/cdc37/RocCOR LRRK2 1867-2161LRRK2 (Homo sapiens)Baculovirus WT aa1867-2161
40398pBACgus4x/cdc37/RocCOR LRRK2 1867-2176LRRK2 (Homo sapiens)Baculovirus WT aa1867-2176
40399pBACgus N-His/GSTGST (Synthetic)Baculovirus WT
40402pBACgus4x/Parkin/RocCOR LRRK2 1867-2176LRRK2 (Homo sapiens)Baculovirus WT aa1867-2176
40403pBACgus N-His/GST/LRRK2 1867-2186LRRK2 (Homo sapiens)Baculovirus WT aa1867-2186
40404pBACgus N-His/GST/LRRK2 1867-2141LRRK2 (Homo sapiens)Baculovirus WT aa1867-2141
40405pBACgus N-His/GST/LRRK2 1867-2161LRRK2 (Homo sapiens)Baculovirus WT aa1867-2161
40406pBACgus N-His/GST/LRRK2 1867-2176LRRK2 (Homo sapiens)Baculovirus WT aa1867-2176
40407LRRK2_1867_2143LRRK2 (Homo sapiens)Baculovirus WT aa1867_2143
40408LRRK2_1867_2136LRRK2 (Homo sapiens)Baculovirus WT aa1867_2136
40409LRRK2_1867_2134LRRK2 (Homo sapiens)Baculovirus WT aa1867_2134
40410LRRK2_1867_2170LRRK2 (Homo sapiens)Baculovirus WT aa1867_2170
40411LRRK2_1867_2161_F1908YLRRK2 (Homo sapiens)Baculovirus aa1867_2161, F1908Y
40412LRRK2_1867_2161_V1922T_C2024S_C2025S_F2079Y_L2115ALRRK2 (Homo sapiens)Baculovirus aa1867-2161; V1922T, C2024S, C2025S, F2079Y, L2115A
40413LRRK2_1867_2161_F1908A_L1936R_V2048S_F2079GLRRK2 (Homo sapiens)Baculovirus aa1867-2161; F1908A, L1936R, V2048S, F2079G
40414LRRK2_1867_2161_F1908A_F2079GLRRK2 (Homo sapiens)Baculovirus aa1867_2161; F1908A, F2079G
40415LRRK2_1867_2161_F1908A_V2048S_I2131QLRRK2 (Homo sapiens)Baculovirus aa1867_2161; F1908A, V2048S, I2131Q
40416LRRK2_1867_2161_I2131QLRRK2 (Homo sapiens)Baculovirus aa1867_2161; I2131Q
40417LRRK2_1867_2161_V2048S_F2079GLRRK2 (Homo sapiens)Baculovirus aa1867_2161; V2048S, F2079G
40418LRRK2_1867_2161_F1908A_L1936R_V2048S_I2131QLRRK2 (Homo sapiens)Baculovirus aa1867-2161; F1908A, L1936R, V2048S, I2131Q
40419LRRK2_1867_2161_V2048SLRRK2 (Homo sapiens)Baculovirus aa1867_2161; V2048S
40420LRRK2_1867_2161_F1908A_L1936R_V2048SLRRK2 (Homo sapiens)Baculovirus aa1867_2161; F1908A, L1936R, V2048S
40421LRRK2_1867_2161_F1908A_L1936R_I2131QLRRK2 (Homo sapiens)Baculovirus aa1867_2161; F1908A, L1936R, I2131Q
40422LRRK2_1867_2161_F1908A_L1936R_F2079G_I2131QLRRK2 (Homo sapiens)Baculovirus aa1867_2161; F1908A, L1936R, F2079G, I2131Q
40423LRRK2_1867_2161_F1908A_L1936R_V2048S_F2079G_I2131QLRRK2 (Homo sapiens)Baculovirus aa1867_2161; F1908A, L1936R, V2048S, F2079G, I2131Q
40424LRRK2_1867_2161_L1914N_V2048TLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1914N, V2048T
40425LRRK2_1867_2161_L1914N_V2048T_F2083Y_I2131SLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1914N, V2048T, F2083Y, I2131S
40426LRRK2_1867_2161_L1914N_L1936Y_V2048T_F2083Y_I2131SLRRK2 (Homo sapiens)Baculovirus 1867_2161; L1914N, L1936Y, V2048T, F2083Y, I2131S
40427pEMB017 NP_9409803_LRRK2_1867_2161_L1914N_V2048T_F2083YLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1914N, V2048T, F2083Y
40428LRRK2_1867_2161_L1914A_L1976Y_C2024S_C2025S_I2049Q_F2083ALRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1914A, L1976Y, C2024S, C2025S, I2049Q, F2083A
40430LRRK2_1867_2161_L1914A_C2024S_C2025S_I2049QLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1914A, C2024S, C2025S, I2049Q
40431LRRK2_1867_2161_L1914A_L1976YLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1914A, L1976Y
40432LRRK2_1867_2161_L1916A_L1936R_L2005N_V2074Y_L2092YLRRK2 (Homo sapiens)Baculovirus aa1867-2161; L1916A, L1936R, L2005N, V2074Y, L2092Y
40433LRRK2_1867_2161_L1914A_L1976Y_I2049Q_F2083ALRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1914A, L1976Y, I2049Q, F2083A
40434LRRK2_1867_2161_L1914A_L1976Y_I2049QLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1914A, L1976Y, I2049Q
40435LRRK2_1867_2161_L1976Y_I2049QLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1976Y, I2049Q
40436LRRK2_1867_2161_L1914ALRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1914A
40438LRRK2_1867_2161_L1916N_L1936R_L1976A_I2049A_L2092NLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1916N, L1936R, L1976A, I2049A, L2092N
40439LRRK2_1867_2161_L1916N_L1976A_I2049A_V2074A_L2092NLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1916N, L1976A, I2049A, V2074A, L2092N
40440LRRK2_1867_2161_V1922D_V2074A_L2115WLRRK2 (Homo sapiens)Baculovirus aa1867_2161; V1922D, V2074A, L2115W
40441LRRK2_1867_2161_V1922D_L2005R_V2074A_L2115WLRRK2 (Homo sapiens)Baculovirus aa1867_2161; V1922D, L2005R, V2074A, L2115W
40442LRRK2_1867_2161_V1922D_L2005R_V2048SLRRK2 (Homo sapiens)Baculovirus aa1867_2161; V1922D, L2005R, V2048S
40443LRRK2_1867_2161_V1922D_L2005R_V2074ALRRK2 (Homo sapiens)Baculovirus aa1867_2161; V1922D, L2005R, V2074A
40444LRRK2_1867_2161_V1922D_V2048SLRRK2 (Homo sapiens)Baculovirus aa1867_2161; V1922D, V2048S
40445LRRK2_1867_2161_F1908Y_L2115ALRRK2 (Homo sapiens)Baculovirus aa1867_2161; F1908Y, L2115A
40446LRRK2_1867_2161_F1908A_V2048SLRRK2 (Homo sapiens)Baculovirus aa1867_2161; F1908A, V2048S
40447LRRK2_1867_2161_F1908A_V2048S_F2079G_I2131QLRRK2 (Homo sapiens)Baculovirus aa1867_2161; F1908A, V2048S, F2079G, I2131Q
40448LRRK2_1867_2161_I2131SLRRK2 (Homo sapiens)Baculovirus aa1867_2161; I2131S
40449LRRK2_1867_2161_L1936Y_I2131SLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1936Y, I2131S
40451LRRK2_1867_2161_L1976Y_I2049Q_F2083ALRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1976Y, I2049Q, F2083A
40452LRRK2_1867_2161_L1914A_L1976Y_C2024S_C2025S_I2049QLRRK2 (Homo sapiens)Baculovirus aa1867_2161; L1914A, L1976Y, C2024S, C2025S, I2049Q
40499pEMB018 / LRRK2_BC_1300_2136LRRK2 (Homo sapiens)Baculovirus WT; aa1300_2136
40500pEMB018 / LRRK2_BC_1300_2148LRRK2 (Homo sapiens)Baculovirus WT; aa1300_2148
40501pEMB018 / LRRK2_BC_1300_2170LRRK2 (Homo sapiens)Baculovirus WT; aa300_2170
40502pEMB018 / LRRK2_BC_1300_2210LRRK2 (Homo sapiens)Baculovirus WT; aa1300_2210
40503pEMB018 / LRRK2_BC_1309_2136LRRK2 (Homo sapiens)Baculovirus WT; aa1309_2136
40504pEMB018 / LRRK2_BC_1309_2170LRRK2 (Homo sapiens)Baculovirus WT; aa1309_2170
40505pEMB018 / LRRK2_BC_1309_2187LRRK2 (Homo sapiens)Baculovirus WT; aa1309_2187
40506pEMB018 / LRRK2_BC_1309_2210LRRK2 (Homo sapiens)Baculovirus WT; aa1309_2210
40507pEMB018 / LRRK2_BC_1320_2210LRRK2 (Homo sapiens)Baculovirus WT; aa1320_2210
40509pEMB018 / LRRK2_BC_1333_2148LRRK2 (Homo sapiens)Baculovirus WT; aa1333_2148
40510pEMB018 / LRRK2_BC_1333_2187LRRK2 (Homo sapiens)Baculovirus WT; aa1333_2187
40511pEMB018 / LRRK2_BC_1333_2210LRRK2 (Homo sapiens)Baculovirus WT; aa1333_2210
40512pEMB018 / LRRK2_BC_1500_2136LRRK2 (Homo sapiens)Baculovirus WT; aa1500_2136
40513pEMB018 / LRRK2_BC_1500_2148LRRK2 (Homo sapiens)Baculovirus WT; aa1500_2148
40514pEMB018 / LRRK2_BC_1500_2170LRRK2 (Homo sapiens)Baculovirus WT; aa1500_2170
40515pEMB018 / LRRK2_BC_1500_2187LRRK2 (Homo sapiens)Baculovirus WT; aa1500_2187
40516pEMB018 / LRRK2_BC_1500_2210LRRK2 (Homo sapiens)Baculovirus WT; aa1500_2210
40517pEMB018 / LRRK2_BC_1510_2170LRRK2 (Homo sapiens)Baculovirus WT; aa1510_2170
40518pEMB018 / LRRK2_BC_1510_2187LRRK2 (Homo sapiens)Baculovirus WT; aa1510_2187
40519pEMB018 / LRRK2_BC_1520_2148LRRK2 (Homo sapiens)Baculovirus WT; aa1520_2148
40520pEMB018 / LRRK2_BC_1520_2170LRRK2 (Homo sapiens)Baculovirus WT; aa1520_2170
40521pEMB018 / LRRK2_BC_1520_2187LRRK2 (Homo sapiens)Baculovirus WT; aa1520_2187
40522pEMB018 / LRRK2_BC_1520_2210LRRK2 (Homo sapiens)Baculovirus WT; aa1520_2210
40523pEMB018 / LRRK2_BC_1309_2527_M2397TLRRK2 (Homo sapiens)Baculovirus aa1309_2527; M2397T
40525pEMB018 / LRRK2_BC_1300_2232LRRK2 (Homo sapiens)Baculovirus WT; aa1300_2232
40526pEMB018 / LRRK2_BC_1309_2232LRRK2 (Homo sapiens)Baculovirus WT; aa1309_2232
40527pEMB018 / LRRK2_BC_1333_2232LRRK2 (Homo sapiens)Baculovirus WT; aa1333_2232
40528pEMB018 / LRRK2_BC_1300_2350LRRK2 (Homo sapiens)Baculovirus WT; aa1300_2350
40529pEMB018 / LRRK2_BC_1309_2350LRRK2 (Homo sapiens)Baculovirus WT; aa1309_2350
40530pEMB018 / LRRK2_BC_1500_2350LRRK2 (Homo sapiens)Baculovirus WT; aa1500_2350
40531pEMB018 / LRRK2_BC_1500_2232LRRK2 (Homo sapiens)Baculovirus WT; aa1500_2232
40532pEMB018 / LRRK2_BC_1847_2232LRRK2 (Homo sapiens)Baculovirus WT; aa1847_2232
40533pEMB018 / LRRK2_BC_1847_2350LRRK2 (Homo sapiens)Baculovirus WT; aa1847_2350
40535pEMB018 / LRRK2_BC_1882_2350LRRK2 (Homo sapiens)Baculovirus WT; aa1882_2350
40536pEMB018 / LRRK2_BC_1847_2527_M2397TLRRK2 (Homo sapiens)Baculovirus aa1847_2527; M2397T
40537pEMB018 / LRRK2_BC_1867_2527_M2397TLRRK2 (Homo sapiens)Baculovirus aa1867_2527; M2397T
40538pEMB018 / LRRK2_BC_1882_2527_M2397TLRRK2 (Homo sapiens)Baculovirus aa1882_2527; M2397T
40539pEMB018 / LRRK2_BC_1185_2136LRRK2 (Homo sapiens)Baculovirus WT; aa1185_2136
40540pEMB52/Bag5_1_447Bag5 (Homo sapiens)Baculovirus WT; aa1-447
40541pEMB52/14-3-3_1_247YWHAG (Homo sapiens)Baculovirus WT; aa1-247
40542pEMB52/Dvl1_1_670DVL1 (Homo sapiens)Baculovirus WT
40543pEMB52/Armadillo_34_373LRRK2Baculovirus WT
40544pEMB52/Horse_34_374LRRK2Baculovirus WT
40545pEMB52/Turkey_34_374LRRK2Baculovirus WT
40549pEMB52 / Human_LRRK1_1_360LRRK1 (Homo sapiens)Baculovirus WT
40550pEMB52 / Anemone_LRRK2_1_334LRRK2Baculovirus WT
40551pEMB52 / Urchin_LRRK2_1_335LRRK2Baculovirus WT
40552pEMB12/LRRK2_BC_1847_2170_P1865CCCLRRK2 (Homo sapiens)Baculovirus WT; aa1847_2170
40553pEMB12 / LRRK2_BC_1847_2210LRRK2 (Homo sapiens)Baculovirus WT; aa1847_2210
40554pEMB44 / Lancelet_LRRK2_1_319LRRK2Baculovirus WT
40555pEMB44 / Mouse_LRRK2_1_342Lrrk2 (Mus musculus)Baculovirus WT
40556pEMB44 / Zebrafish_LRRK2_1_338lrrk2 (Danio rerio)Baculovirus WT
40557pEMB36(revcomp)/Dvl1_1_670DVL1 (Homo sapiens)Baculovirus WT; aa1_670
40561pEMB403 Urchin_LRRK2_1_335LRRK2Baculovirus WT
40564pEMB12 LRRK2_BC_1847_2210_K1906MLRRK2 (Homo sapiens)Baculovirus aa1847_2210; K1906M
40565 pEMB52 LRRK2_BC_1520_2210_I2211ENLYFQGWSHPQFEKLRRK2 (Homo sapiens)Baculovirus WT; aa1520_2210
40566pEMB52 LRRK2_BC_1520_2148_I2149ENLYFQGWSHPQFEKLRRK2 (Homo sapiens)Baculovirus WT; aa1520_2148
40567 pEMB018 LRRK2_BC_1520_2148_K1906MLRRK2 (Homo sapiens)Baculovirus aa1520_2148; K1906M
51437human Alpha-synucleinAlpha-synuclein (Homo sapiens)Cloning
51486pET21a-alpha-synucleinalpha-synuclein (Homo sapiens)Bacterial Expression
51488pET3a-His-DJ1DJ1 (Homo sapiens)Bacterial Expression
51489pET3a-DJ1DJ1 (Homo sapiens)Bacterial Expression
89073pD434-SR mouse Alpha-synucleinAlpha-synuclein (Mus musculus)Bacterial Expression
89074pD454-SR rat Alpha-synucleinAlpha-synuclein (Rattus norvegicus)Bacterial Expression
89075pD454-SR mouse Alpha-synucleinAlpha-synuclein (Mus musculus)Bacterial Expression
89076pD434-SR rat Alpha-synucleinAlpha-synuclein (Rattus norvegicus)Bacterial Expression