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B. subtilis Strain Collection
(Kit # 1000000115, 1000000116 )

Depositing Labs: Carol Gross

Bacillus subtilis is a key Gram positive model bacterium used to study diverse research topics such as chromosome replication, competence, or sporulation. Despite being well-studied and having a fully sequenced genome, there are still many unknown or poorly characterized genes. To facilitate better understanding of individual B. subtilis genes, the Gross lab at UCSF has constructed 2 ordered libraries:

Kit 1000000115

B. subtilis Single Gene Deletion Library--Kanamycin is an arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.
This kit will be sent as bacterial glycerol stocks in a 384-well plate format.

Kit 1000000116

B. subtilis CRISPRi Essential Gene Knockdown Library is an arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.
This kit will be sent as bacterial glycerol stocks in a 384-well plate format.

Ordering

Item Catalog # Description Quantity Price (USD)
Kit 1000000115 B. subtilis Single Gene Deletion Library--Kanamycin 1 $5000 Add to Cart
Kit 1000000116 B. subtilis CRISPRi Essential Gene Knockdown Library 1 $1050 Add to Cart
This material is available to academics and nonprofits only.

Description

Publication List for Kits:

Kit 1000000115: B. subtilis Single Gene Deletion Library--Kanamycin

A comprehensive single-gene deletion library of the model bacterium B. subtilis 168. The entire protein coding region (not including start and stop codons) for every non-essential gene was replaced with a kanamycin cassette. The library was designed to allow for easy removal of the kan-cassette via Cre recombination and includes barcodes and universal priming sites to enable high-throughput approaches.

The collection includes 3984 knockout strains arrayed in 384-well format (listed in 'Kit Contents' tab or download plate maps under 'Protocols & Resources'). Strains can be grown at 37C. Kanamycin (7.5ug/mL) is recommended for plate selection; 3ug/mL kanamycin can be used for liquid media, but is not necessarily required.

Kit #1000000116: B. subtilis CRISPRi Essential Gene Knockdown Library

A comprehensive arrayed knockdown library targeting all the essential genes from the model bacterium B. subtilis 168. The library was designed using CRISPR interference (CRISPRi) technology and includes a xylose-inducible promoter driving dCas9 paired with a single sgRNA driven by a strong constitutive promoter targeting the 5’ end of each essential gene.

The collection includes 384 CRISPRi strains arrayed in 384-well format (listed in 'Kit Contents tab' or download plate maps under 'Protocols & Resources'). Strains can be grown at 37C under Chloramphenicol selection (6ug/mL).

Resources

These materials are only being distributed by Addgene as full collections. Please contact the Bacillus Genetic Stock Center to obtain individual strains included in Kit 1000000115, Kit 1000000116, or to request strains from the erythromycin-knockout library (not available through Addgene). Please note that Addgene has not sequence-verified these strains and the collections are distributed "as is" based on the depositor's information. We strongly recommend verifying the strains prior to use.

Strain and Plate Data

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The B. subtilis Strain Collection was a gift from Carol Gross (Addgene kit #).”

For your Reference section:

Kit #1000000115: B. subtilis Single Gene Deletion Library--Kanamycin

Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis. Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA. Cell Syst. 2017 Mar 22;4(3):291-305.e7. doi: 10.1016/j.cels.2016.12.013. PubMed PMID: 28189581.

Kit #1000000116: B. subtilis CRISPRi Essential Gene Knockdown Library

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CHS, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA. Cell. 2016 Jun 2;165(6):1493-1506. doi: 10.1016/j.cell.2016.05.003. PubMed PMID: 27238023.

B. subtilis Single Gene Deletion Library--Kanamycin - #1000000115

Icon BSubtilis Single Gene Deletion Library Data (669.3 KB)

B. subtilis CRISPRi Essential Gene Knockdown Library - #1000000116

Well Number Name
A01 Strain name: CAG74399_1; Gene target: no_sgRNA
A02 Strain name: CAG74399_21; Gene target: no_sgRNA
A03 Strain name: CAG74399_2; Gene target: no_sgRNA
A04 Strain name: CAG74399_22; Gene target: no_sgRNA
A05 Strain name: CAG74399_3; Gene target: no_sgRNA
A06 Strain name: CAG74399_23; Gene target: no_sgRNA
A07 Strain name: CAG74399_4; Gene target: no_sgRNA
A08 Strain name: CAG74399_24; Gene target: no_sgRNA
A09 Strain name: CAG74399_5; Gene target: no_sgRNA
A10 Strain name: CAG74399_25; Gene target: no_sgRNA
A11 Strain name: CAG74399_6; Gene target: no_sgRNA
A12 Strain name: CAG74399_26; Gene target: no_sgRNA
A13 Strain name: CAG74399_7; Gene target: no_sgRNA
A14 Strain name: CAG74399_27; Gene target: no_sgRNA
A15 Strain name: CAG74399_8; Gene target: no_sgRNA
A16 Strain name: CAG74399_28; Gene target: no_sgRNA
A17 Strain name: CAG74399_9; Gene target: no_sgRNA
A18 Strain name: CAG74399_29; Gene target: no_sgRNA
A19 Strain name: CAG74399_10; Gene target: no_sgRNA
A20 Strain name: CAG74399_30; Gene target: no_sgRNA
A21 Strain name: CAG74399_11; Gene target: no_sgRNA
A22 Strain name: CAG74399_31; Gene target: no_sgRNA
A23 Strain name: CAG74399_12; Gene target: no_sgRNA
A24 Strain name: CAG74399_32; Gene target: no_sgRNA
B01 Strain name: CAG74399_40; Gene target: no_sgRNA
B02 Strain name: BEC22600; Gene target: aroE
B03 Strain name: BEC22710; Gene target: aroF
B04 Strain name: BEC08930; Gene target: cspR
B05 Strain name: BEC30540; Gene target: asnB
B06 Strain name: BEC14180; Gene target: dapH
B07 Strain name: BEC22450; Gene target: cca
B08 Strain name: BEC15910; Gene target: fabG
B09 Strain name: BEC15700; Gene target: coaBC
B10 Strain name: BEC27940; Gene target: rpmA
B11 Strain name: BEC25210; Gene target: dnaG
B12 Strain name: BEC10130; Gene target: hemH
B13 Strain name: BEC14190; Gene target: dapL
B14 Strain name: BEC10140; Gene target: hemY
B15 Strain name: BEC00620; Gene target: divIC
B16 Strain name: BEC31650; Gene target: mrpF
B17 Strain name: BEC00010; Gene target: dnaA
B18 Strain name: BEC17390; Gene target: nrdF
B19 Strain name: BEC28990; Gene target: dnaB
B20 Strain name: BEC23130; Gene target: resC
B21 Strain name: BEC29230; Gene target: dnaE
B22 Strain name: BEC15920; Gene target: acpP
B23 Strain name: BEC22750; Gene target: ubiE
B24 Strain name: CAG74399_61; Gene target: no_sgRNA
C01 Strain name: CAG74399_13; Gene target: no_sgRNA
C02 Strain name: BEC28980; Gene target: dnaI
C03 Strain name: BEC06720; Gene target: yerQ
C04 Strain name: BEC01780; Gene target: glmS
C05 Strain name: BEC32710; Gene target: sufC
C06 Strain name: BEC00500; Gene target: glmU
C07 Strain name: BEC31610; Gene target: mrpB
C08 Strain name: BEC25270; Gene target: glyQ
C09 Strain name: BEC15900; Gene target: fabD
C10 Strain name: BEC27410; Gene target: alaS
C11 Strain name: BEC14530; Gene target: rnjA
C12 Strain name: BEC28150; Gene target: hemC
C13 Strain name: BEC16500; Gene target: tsf
C14 Strain name: BEC04560; Gene target: ddl
C15 Strain name: BEC21810; Gene target: dfrA
C16 Strain name: BEC27500; Gene target: mnmA
C17 Strain name: BEC32670; Gene target: sufB
C18 Strain name: BEC29200; Gene target: accA
C19 Strain name: BEC01440; Gene target: rplQ
C20 Strain name: BEC24340; Gene target: accC
C21 Strain name: BEC28080; Gene target: folC
C22 Strain name: BEC01370; Gene target: adk
C23 Strain name: BEC19360; Gene target: odhB
C24 Strain name: CAG74399_33; Gene target: no_sgRNA
D01 Strain name: CAG74399_41; Gene target: no_sgRNA
D02 Strain name: BEC01500; Gene target: rpsI
D03 Strain name: BEC16550; Gene target: dxr
D04 Strain name: BEC01100; Gene target: rpsL
D05 Strain name: BEC24270; Gene target: dxs
D06 Strain name: BEC01410; Gene target: rpsM
D07 Strain name: BEC22840; Gene target: engA
D08 Strain name: BEC01200; Gene target: rpsS
D09 Strain name: BEC33900; Gene target: eno
D10 Strain name: BEC_TRNA_23; Gene target: trnH
D11 Strain name: BEC25290; Gene target: era
D12 Strain name: BEC04620.50.1; Gene target: acpS50_1
D13 Strain name: BEC11340; Gene target: fabF
D14 Strain name: BEC15240; Gene target: divIB
D15 Strain name: BEC15730; Gene target: fmt
D16 Strain name: BEC22440; Gene target: birA
D17 Strain name: BEC22780; Gene target: folE
D18 Strain name: BEC13300; Gene target: mgtE
D19 Strain name: BEC00790; Gene target: folK
D20 Strain name: BEC27880; Gene target: nifS
D21 Strain name: BEC15150; Gene target: ftsL
D22 Strain name: BEC14580; Gene target: pdhA
D23 Strain name: BEC16530; Gene target: uppS
D24 Strain name: CAG74399_62; Gene target: no_sgRNA
E01 Strain name: CAG74399_14; Gene target: no_sgRNA
E02 Strain name: BEC22760; Gene target: hepS
E03 Strain name: BEC32170; Gene target: dapF
E04 Strain name: BEC25560; Gene target: holA
E05 Strain name: BEC28390; Gene target: racE
E06 Strain name: BEC04640; Gene target: alrA
E07 Strain name: BEC03150; Gene target: aroK
E08 Strain name: BEC09540; Gene target: plsC
E09 Strain name: BEC35720; Gene target: tagF
E10 Strain name: BEC35660; Gene target: mnaA
E11 Strain name: CAG74399_15; Gene target: rpsN
E12 Strain name: BEC27550; Gene target: aspS
E13 Strain name: BEC30790; Gene target: menE
E14 Strain name: BEC16540; Gene target: cdsA
E15 Strain name: BEC31630; Gene target: mrpD
E16 Strain name: BEC22890; Gene target: cmk
E17 Strain name: BEC22490; Gene target: dapB
E18 Strain name: BEC00900; Gene target: ispD
E19 Strain name: BEC06680; Gene target: gatA
E20 Strain name: BEC01380; Gene target: mapA
E21 Strain name: BEC36760; Gene target: murAA
E22 Strain name: BEC00380; Gene target: metS
E23 Strain name: BEC28190; Gene target: engB
E24 Strain name: CAG74399_34; Gene target: no_sgRNA
F01 Strain name: CAG74399_42; Gene target: no_sgRNA
F02 Strain name: BEC28850; Gene target: rplT
F03 Strain name: BEC01770; Gene target: glmM
F04 Strain name: BEC01290; Gene target: rpsNA
F05 Strain name: BEC36900; Gene target: glyA
F06 Strain name: BEC14840.27.2; Gene target: ylaN27_2
F07 Strain name: BEC15680; Gene target: gmk
F08 Strain name: BEC14840.87; Gene target: ylaN87
F09 Strain name: BEC22830; Gene target: gpsA
F10 Strain name: BEC14840.189; Gene target: ylaN189
F11 Strain name: BEC06030; Gene target: groEL
F12 Strain name: BEC14840.192; Gene target: ylaN192
F13 Strain name: BEC06020; Gene target: groES
F14 Strain name: CAG74399_63; Gene target: no_sgRNA
F15 Strain name: BEC00090; Gene target: guaB
F16 Strain name: BEC12519; Gene target: yjzJ
F17 Strain name: BEC00070; Gene target: gyrA
F18 Strain name: BEC23230; Gene target: ypuF
F19 Strain name: BEC28170; Gene target: hemA
F20 Strain name: CAG74399_64; Gene target: no_sgRNA
F21 Strain name: BEC12230; Gene target: yjjA
F22 Strain name: BEC09510; Gene target: yhdL
F23 Strain name: BEC10120; Gene target: hemE
F24 Strain name: CAG74399_65; Gene target: no_sgRNA
G01 Strain name: CAG74399_16; Gene target: no_sgRNA
G02 Strain name: BEC28030; Gene target: mreB
G03 Strain name: BEC15890; Gene target: plsX
G04 Strain name: BEC28020; Gene target: mreC
G05 Strain name: BEC00310; Gene target: holB
G06 Strain name: BEC28010; Gene target: mreD
G07 Strain name: BEC18100; Gene target: parC
G08 Strain name: BEC15200; Gene target: murD
G09 Strain name: BEC16510; Gene target: pyrH
G10 Strain name: BEC35750; Gene target: tagA
G11 Strain name: BEC28640; Gene target: pheS
G12 Strain name: BEC17370; Gene target: nrdI
G13 Strain name: BEC29060; Gene target: coaE
G14 Strain name: BEC22420; Gene target: panC
G15 Strain name: BEC01130; Gene target: tufA
G16 Strain name: BEC06070; Gene target: ydiP
G17 Strain name: BEC27960; Gene target: rplU
G18 Strain name: BEC15160; Gene target: pbpB
G19 Strain name: BEC18070; Gene target: yneS
G20 Strain name: BEC00190; Gene target: dnaX
G21 Strain name: BEC27560; Gene target: hisS
G22 Strain name: BEC16920; Gene target: pgsA
G23 Strain name: BEC35530; Gene target: tagO
G24 Strain name: CAG74399_35; Gene target: no_sgRNA
H01 Strain name: CAG74399_43; Gene target: no_sgRNA
H02 Strain name: BEC07840; Gene target: yfkN
H03 Strain name: BEC15430; Gene target: ileS
H04 Strain name: BEC16030; Gene target: trmD
H05 Strain name: BEC28870; Gene target: infC
H06 Strain name: BEC16760; Gene target: dapG
H07 Strain name: BEC00460; Gene target: ipk
H08 Strain name: BEC14850; Gene target: ftsW
H09 Strain name: BEC00910; Gene target: ispF
H10 Strain name: CAG74399_66; Gene target: ftsZ
H11 Strain name: BEC25070; Gene target: ispG
H12 Strain name: BEC00920; Gene target: gltX
H13 Strain name: BEC25160; Gene target: ispH
H14 Strain name: BEC00060; Gene target: gyrB
H15 Strain name: BEC30320; Gene target: leuS
H16 Strain name: BEC00820; Gene target: lysS
H17 Strain name: BEC21320; Gene target: yomL
H18 Strain name: BEC30770; Gene target: mntA
H19 Strain name: BEC38490; Gene target: menA
H20 Strain name: BEC15190; Gene target: mraY
H21 Strain name: BEC30780; Gene target: menC
H22 Strain name: BEC15180; Gene target: murE
H23 Strain name: BEC30820; Gene target: menD
H24 Strain name: CAG74399_67; Gene target: no_sgRNA
I01 Strain name: CAG74399_17; Gene target: no_sgRNA
I02 Strain name: BEC35290; Gene target: prfB
I03 Strain name: BEC15220; Gene target: murG
I04 Strain name: BEC37150; Gene target: pyrG
I05 Strain name: BEC00020; Gene target: dnaN
I06 Strain name: BEC41050; Gene target: rnpA
I07 Strain name: BEC05940; Gene target: gcp
I08 Strain name: BEC_MISC_RNA_35; Gene target: rnpB
I09 Strain name: BEC29210; Gene target: accD
I10 Strain name: BEC01190; Gene target: rplB
I11 Strain name: BEC01320.2; Gene target: rplR-2
I12 Strain name: BEC01050; Gene target: rplL
I13 Strain name: BEC01330; Gene target: rpsE
I14 Strain name: BEC01390; Gene target: infA
I15 Strain name: BEC00770; Gene target: sul
I16 Strain name: BEC16630; Gene target: infB
I17 Strain name: BEC29670; Gene target: tyrS
I18 Strain name: BEC32680; Gene target: iscU
I19 Strain name: BEC23840; Gene target: yqjK
I20 Strain name: BEC06620; Gene target: ligA
I21 Strain name: BEC25260; Gene target: glyS
I22 Strain name: BEC06670; Gene target: gatC
I23 Strain name: BEC00940; Gene target: cysS
I24 Strain name: CAG74399_36; Gene target: no_sgRNA
J01 Strain name: CAG74399_44; Gene target: no_sgRNA
J02 Strain name: BEC03130; Gene target: nadE
J03 Strain name: BEC40400; Gene target: walK
J04 Strain name: BEC17380; Gene target: nrdE
J05 Strain name: CAG74399_45; Gene target: yqaE
J06 Strain name: BEC16600; Gene target: nusA
J07 Strain name: BEC12520; Gene target: xkdB
J08 Strain name: BEC16580; Gene target: polC
J09 Strain name: BEC31600; Gene target: mrpA
J10 Strain name: BEC11610; Gene target: ppnKB
J11 Strain name: BEC31660; Gene target: mrpG
J12 Strain name: BEC37010; Gene target: prfA
J13 Strain name: BEC15230; Gene target: murB
J14 Strain name: BEC38120; Gene target: rodA
J15 Strain name: BEC29790; Gene target: murC
J16 Strain name: BEC00130; Gene target: serS
J17 Strain name: BEC32110; Gene target: yumC
J18 Strain name: BEC14840.27.1; Gene target: ylaN27_1
J19 Strain name: BEC38690; Gene target: yxlC
J20 Strain name: BEC15290.226; Gene target: ftsZ226
J21 Strain name: BEC06610; Gene target: pcrA
J22 Strain name: BEC09950; Gene target: prsA
J23 Strain name: BEC33910; Gene target: pgm
J24 Strain name: CAG74399_68; Gene target: no_sgRNA
K01 Strain name: CAG74399_18; Gene target: no_sgRNA
K02 Strain name: BEC31640; Gene target: mrpE
K03 Strain name: BEC01340; Gene target: rpmD
K04 Strain name: BEC25640; Gene target: nadD
K05 Strain name: BEC35710; Gene target: tagG
K06 Strain name: BEC14000; Gene target: patA
K07 Strain name: BEC28130; Gene target: hemB
K08 Strain name: BEC40550; Gene target: ppaC
K09 Strain name: BEC16770; Gene target: dapA
K10 Strain name: BEC01230; Gene target: rplP
K11 Strain name: BEC23220; Gene target: scpA
K12 Strain name: BEC00680; Gene target: hprT
K13 Strain name: BEC06690; Gene target: gatB
K14 Strain name: BEC29660; Gene target: rpsD
K15 Strain name: BEC16520; Gene target: frr
K16 Strain name: BEC01250; Gene target: rpsQ
K17 Strain name: BEC22790; Gene target: hbs
K18 Strain name: BEC40890; Gene target: rpsR
K19 Strain name: BEC32690; Gene target: sufS
K20 Strain name: BEC35300; Gene target: secA
K21 Strain name: BEC31620; Gene target: mrpC
K22 Strain name: BEC21490; Gene target: sunI
K23 Strain name: BEC01120; Gene target: fusA
K24 Strain name: CAG74399_37; Gene target: no_sgRNA
L01 Strain name: CAG74399_46; Gene target: no_sgRNA
L02 Strain name: BEC01320.105; Gene target: rplR105
L03 Strain name: BEC05920; Gene target: ydiC
L04 Strain name: BEC04620.50.2; Gene target: acpS50_2
L05 Strain name: BEC06060; Gene target: ydiO
L06 Strain name: BEC26350.101; Gene target: yqaE101
L07 Strain name: BEC00510; Gene target: prs
L08 Strain name: BEC06811.130; Gene target: yezG130
L09 Strain name: BEC16050; Gene target: rbgA
L10 Strain name: BEC24330; Gene target: yqhY
L11 Strain name: BEC23140; Gene target: resB
L12 Strain name: BEC03100; Gene target: ycgG
L13 Strain name: BEC16670; Gene target: ribC
L14 Strain name: BEC24310; Gene target: folD
L15 Strain name: CAG74399_47; Gene target: yezG
L16 Strain name: BEC15280; Gene target: ftsA
L17 Strain name: BEC01160; Gene target: rplC
L18 Strain name: BEC30800; Gene target: menB
L19 Strain name: BEC01170; Gene target: rplD
L20 Strain name: BEC22740; Gene target: hepT
L21 Strain name: BEC01280; Gene target: rplE
L22 Strain name: BEC16750; Gene target: asd
L23 Strain name: BEC01310; Gene target: rplF
L24 Strain name: CAG74399_69; Gene target: no_sgRNA
M01 Strain name: CAG74399_19; Gene target: no_sgRNA
M02 Strain name: BEC01270; Gene target: rplX
M03 Strain name: BEC15980; Gene target: ffh
M04 Strain name: BEC01080; Gene target: rpoC
M05 Strain name: BEC24350; Gene target: accB
M06 Strain name: BEC01150; Gene target: rpsJ
M07 Strain name: BEC33940; Gene target: gapA
M08 Strain name: BEC01420; Gene target: rpsK
M09 Strain name: BEC01000; Gene target: secE
M10 Strain name: BEC30550; Gene target: metK
M11 Strain name: BEC00780; Gene target: folB
M12 Strain name: BEC32700; Gene target: sufD
M13 Strain name: BEC16490; Gene target: rpsB
M14 Strain name: BEC35760; Gene target: tagB
M15 Strain name: BEC18090; Gene target: parE
M16 Strain name: BEC34790; Gene target: trxB
M17 Strain name: BEC40440; Gene target: dnaC
M18 Strain name: BEC28090; Gene target: valS
M19 Strain name: BEC22350; Gene target: dnaD
M20 Strain name: BEC12530; Gene target: xkdC
M21 Strain name: BEC36410; Gene target: mbl
M22 Strain name: BEC05100; Gene target: yddT
M23 Strain name: BEC28500; Gene target: trxA
M24 Strain name: CAG74399_38; Gene target: no_sgRNA
N01 Strain name: CAG74399_48; Gene target: no_sgRNA
N02 Strain name: BEC29050; Gene target: ytbE
N03 Strain name: BEC01490; Gene target: rplM
N04 Strain name: BEC15020; Gene target: coaD
N05 Strain name: BEC16040; Gene target: rplS
N06 Strain name: BEC11420; Gene target: trpS
N07 Strain name: BEC01070; Gene target: rpoB
N08 Strain name: BEC01300; Gene target: rpsH
N09 Strain name: BEC01110; Gene target: rpsG
N10 Strain name: CAG74399_70; Gene target: no_sgRNA
N11 Strain name: BEC16680; Gene target: rpsO
N12 Strain name: CAG74399_71; Gene target: no_sgRNA
N13 Strain name: BEC15990; Gene target: rpsP
N14 Strain name: CAG74399_72; Gene target: no_sgRNA
N15 Strain name: BEC_MISC_RNA_2; Gene target: scr
N16 Strain name: CAG74399_73; Gene target: no_sgRNA
N17 Strain name: BEC25200; Gene target: sigA
N18 Strain name: CAG74399_74; Gene target: no_sgRNA
N19 Strain name: BEC15940; Gene target: smc
N20 Strain name: CAG74399_75; Gene target: no_sgRNA
N21 Strain name: BEC35700; Gene target: tagH
N22 Strain name: CAG74399_76; Gene target: no_sgRNA
N23 Strain name: BEC00670; Gene target: tilS
N24 Strain name: CAG74399_77; Gene target: no_sgRNA
O01 Strain name: CAG74399_20; Gene target: no_sgRNA
O02 Strain name: BEC01040; Gene target: rplJ
O03 Strain name: BEC15930; Gene target: rnc
O04 Strain name: BEC16960; Gene target: ymdA
O05 Strain name: BEC15830; Gene target: yloU
O06 Strain name: BEC17910; Gene target: yneF
O07 Strain name: BEC15710; Gene target: priA
O08 Strain name: BEC25670; Gene target: yqeH
O09 Strain name: BEC28630; Gene target: pheT
O10 Strain name: BEC28650; Gene target: ysgA
O11 Strain name: BEC04570; Gene target: murF
O12 Strain name: BEC35740; Gene target: tagD
O13 Strain name: BEC01260; Gene target: rplN
O14 Strain name: BEC01430; Gene target: rpoA
O15 Strain name: BEC40410; Gene target: walR
O16 Strain name: BEC01220; Gene target: rpsC
O17 Strain name: BEC10660; Gene target: yisB
O18 Strain name: BEC35600; Gene target: tuaB
O19 Strain name: BEC16570; Gene target: proS
O20 Strain name: BEC01360; Gene target: secY
O21 Strain name: BEC33660; Gene target: rghRA
O22 Strain name: BEC40900; Gene target: ssbA
O23 Strain name: BEC00530; Gene target: pth
O24 Strain name: CAG74399_39; Gene target: no_sgRNA
P01 Strain name: CAG74399_49; Gene target: no_sgRNA
P02 Strain name: CAG74399_78; Gene target: no_sgRNA
P03 Strain name: CAG74399_50; Gene target: no_sgRNA
P04 Strain name: CAG74399_79; Gene target: no_sgRNA
P05 Strain name: CAG74399_51; Gene target: no_sgRNA
P06 Strain name: CAG74399_80; Gene target: no_sgRNA
P07 Strain name: CAG74399_52; Gene target: no_sgRNA
P08 Strain name: CAG74399_81; Gene target: no_sgRNA
P09 Strain name: CAG74399_53; Gene target: no_sgRNA
P10 Strain name: CAG74399_82; Gene target: no_sgRNA
P11 Strain name: CAG74399_54; Gene target: no_sgRNA
P12 Strain name: CAG74399_83; Gene target: no_sgRNA
P13 Strain name: CAG74399_55; Gene target: no_sgRNA
P14 Strain name: CAG74399_84; Gene target: no_sgRNA
P15 Strain name: CAG74399_56; Gene target: no_sgRNA
P16 Strain name: CAG74399_85; Gene target: no_sgRNA
P17 Strain name: CAG74399_57; Gene target: no_sgRNA
P18 Strain name: CAG74399_86; Gene target: no_sgRNA
P19 Strain name: CAG74399_58; Gene target: no_sgRNA
P20 Strain name: CAG74399_87; Gene target: no_sgRNA
P21 Strain name: CAG74399_59; Gene target: no_sgRNA
P22 Strain name: CAG74399_88; Gene target: no_sgRNA
P23 Strain name: CAG74399_60; Gene target: no_sgRNA
P24 Strain name: CAG74399_89; Gene target: no_sgRNA
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