Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


Mobius Assembly Vector Toolkit
(Kit # 1000000134 )

Depositing Lab:   Naomi Nakayama

The Mobius Assembly Toolkit is a new Golden Gate Assembly framework, which combines vector toolkit simplicity with high cloning capacity. It is based on a two-level, hierarchical approach and utilizes a low-frequency cutter to reduce domestication requirements. Mobius Assembly embraces the standard overhang designs designated by MoClo, Golden Braid, and Phytobricks and is largely compatible with already available Golden Gate part libraries.

This kit will be shipped as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$400 USD + shipping
Available to academics and nonprofits only.

Original Publication

Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly. Andreou AI, Nakayama N (2018) PLOS ONE 13(1): e0189892. PMID: 29293531 .


Mobius Assembly vector toolkit consists of 16 plasmids: one mUAV, four Level 1 Acceptor Vectors, four Level 2 Acceptor Vectors and seven Auxiliary Plasmids. Mobius Assembly is a Golden Gate Assembly framework which renders both high cloning capacity and vector toolkit simplicity. It is based on a two-level approach which enables a quadruple hierarchical assembly augmentation, it has reduced domestication requirements and employs a chromogenic, visible cloning screening.

Mobius Assembly uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. The standard parts stored in mUAVs are released and fused in a Level 1 reaction to form a TU. Up to four Level 1 TUs can be fused in a Level 2 reaction to form a multi-TU cassette. Switching back and forth between Level 1 and 2 leads to further expansion of multi-TUs according to the geometric sequence: 1, 4, 16, 64, ...

Kit Documentation

Supplemental document:

Mobius_Assembly_Supporting_Materials_and_Protocols.pdf 397.6 KB

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Mobius Assembly Vector Toolkit was a gift from Naomi Nakayama (Addgene kit # 1000000134).”

For your Reference section:

Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly. Andreou AI, Nakayama N (2018) PLOS ONE 13(1): e0189892. PMID: 29293531 .

Mobius Assembly Vector Toolkit - #1000000134

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.


Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key



Well Plasmid Resistance
A / 1 mUAV
A / 2 pMA_Level 1A
A / 3 pMA_Level 1B
A / 4 pMA_Level 1Γ
A / 5 pMA_Level 1Δ
A / 6 pMA_Level 2A
A / 7 pMA_Level 2B
A / 8 pMA_Level 2Γ
A / 9 pMA_Level 2Δ
A / 10 pMA_Auxiliary 1
A / 11 pMA_Auxiliary 2
A / 12 pMA_Auxiliary 3
B / 1 pMA_Auxiliary 4A
B / 2 pMA_Auxiliary 4B
B / 3 pMA_Auxiliary 4Γ
B / 4 pMA_Auxiliary 4Δ
Data calculated @ 2024-06-12

Kit Plate Map - #1000000134

Plate #1

  1 2 3 4 5 6 7 8 9 10 11 12
Content blocked, you may need to disable your ad-blocker.