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MoClo Toolkit
(Kit # 1000000044 )

Depositing Labs:   Sylvestre Marillonnet

This collection contains a set of 95 empty standardized genetic modules that can be used for hierarchical assembly based on the Golden Gate cloning technique. This kit was deposited by Sylvestre Marillonnet's lab and is comprised of plasmids for cloning: promoters, 5' untranslated regions, signal peptides, coding sequences, and terminators. The MoClo kit can be used to assemble any eukaryotic multigene construct in three steps.

This kit consists of 1 plate, and will be shipped to most countries as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLOS ONE . 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PubMed .

Description

This collection contains a set of 95 empty standardized genetic modules that can be used for hierarchical assembly based on the Golden Gate cloning technique. This kit was deposited by Sylvestre Marillonnet's lab and is comprised of plasmids for cloning: promoters, 5' untranslated regions, signal peptides, coding sequences, and terminators. The MoClo kit can be used to assemble any eukaryotic multigene construct in three steps.

PLoS-One-2011-MoClo-Marillonnet-Fig1.png

Image from Weber et al., PLoS One. 2011 Feb 18;6(2):e16765.

Kit Documentation

Documents included with shipment: Icon MoClo Toolkit Plasmid Kit Info (1.2 MB)

Download a ZIP file containing all MoClo Kit plasmid sequences in GenBank format: Icon MoClo Toolkit GenBank files (825.5 KB)

Download a PDF file containing figures illustrating the MoClo Kit constructs: Icon Additional Figures (212.0 KB)

Protocols

See the original PLOS ONE publication ( PubMed ) for detailed protocols for this kit.

Links

For additional information browse the [email protected] resources that have been developed by Nicola Patron and colleagues at The Sainsbury Laboratory:

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The MoClo Toolkit was a gift from Sylvestre Marillonnet (Addgene kit # 1000000044)"

For your Reference section:
A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLOS ONE . 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PubMed .

MoClo Tool Kit - #1000000044

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Kanamycin
Spectinomycin
Ampicillin

Inventory

Well Plasmid Resistance
A / 1 pAGM1311
Kanamycin
A / 2 pICH53399
Spectinomycin
A / 3 pICH41331
Spectinomycin
A / 4 pICH47802
Ampicillin
A / 5 pAGM4723
Kanamycin
A / 6 pICH49255
Ampicillin
A / 7 pICH83977
Ampicillin
A / 8 pAGM8055
Spectinomycin
A / 9 pICH50900
Ampicillin
A / 10 pICH75366
Kanamycin
A / 11 pICH79300
Ampicillin
A / 12 pICH54077
Ampicillin
B / 1 pICH41233
Spectinomycin
B / 2 pICH41246
Spectinomycin
B / 3 pICH47732
Ampicillin
B / 4 pICH47811
Ampicillin
B / 5 pICH41722
Spectinomycin
B / 6 pICH49266
Ampicillin
B / 7 pICH83988
Ampicillin
B / 8 pAGM8067
Spectinomycin
B / 9 pICH50914
Ampicillin
B / 10 pICH75377
Kanamycin
B / 11 pICH79311
Ampicillin
B / 12 pICH89921
Kanamycin
C / 1 pAGM1263
Spectinomycin
C / 2 pICH41264
Spectinomycin
C / 3 pICH47742
Ampicillin
C / 4 pICH47822
Ampicillin
C / 5 pICH41744
Spectinomycin
C / 6 pICH49277
Ampicillin
C / 7 pICH83999
Ampicillin
C / 8 pAGM8079
Spectinomycin
C / 9 pICH50927
Ampicillin
C / 10 pICH75388
Kanamycin
C / 11 pICH54011
Ampicillin
C / 12 pICH82113
Kanamycin
D / 1 pAGM1276
Spectinomycin
D / 2 pICH41276
Spectinomycin
D / 3 pICH47751
Ampicillin
D / 4 pICH47831
Ampicillin
D / 5 pICH41766
Spectinomycin
D / 6 pICH49283
Ampicillin
D / 7 pICH84000
Ampicillin
D / 8 pAGM8081
Spectinomycin
D / 9 pICH50932
Ampicillin
D / 10 pICH79255
Ampicillin
D / 11 pICH54022
Ampicillin
D / 12 pICH82094
Kanamycin
E / 1 pICH41258
Spectinomycin
E / 2 pAGM1251
Spectinomycin
E / 3 pICH47761
Ampicillin
E / 4 pICH47841
Ampicillin
E / 5 pICH41780
Spectinomycin
E / 6 pICH49299
Ampicillin
E / 7 pICH84011
Ampicillin
E / 8 pAGM8093
Spectinomycin
E / 9 pICH75322
Kanamycin
E / 10 pICH79264
Ampicillin
E / 11 pICH54033
Ampicillin
E / 12 pICH86966
Kanamycin
F / 1 pAGM1299
Spectinomycin
F / 2 pAGM1287
Spectinomycin
F / 3 pICH47772
Ampicillin
F / 4 pICH47852
Ampicillin
F / 5 pICH41800
Spectinomycin
F / 6 pICH49300
Ampicillin
F / 7 pICH83955
Ampicillin
F / 8 pICH50872
Ampicillin
F / 9 pICH75334
Kanamycin
F / 10 pICH79277
Ampicillin
F / 11 pICH54044
Ampicillin
F / 12 pICH86988
Kanamycin
G / 1 pAGM1301
Spectinomycin
G / 2 pICH41295
Spectinomycin
G / 3 pICH47781
Ampicillin
G / 4 pICH47861
Ampicillin
G / 5 pICH41822
Spectinomycin
G / 6 pICH49244
Ampicillin
G / 7 pAGM8031
Spectinomycin
G / 8 pICH50881
Ampicillin
G / 9 pICH75344
Kanamycin
G / 10 pICH79289
Ampicillin
G / 11 pICH54055
Ampicillin
G / 12 pAGM9121
Spectinomycin
H / 1 pICH53388
Spectinomycin
H / 2 pICH41308
Spectinomycin
H / 3 pICH47791
Ampicillin
H / 4 pAGM4673
Kanamycin
H / 5 pICH50866
Spectinomycin
H / 6 pICH83966
Ampicillin
H / 7 pAGM8043
Spectinomycin
H / 8 pICH50892
Ampicillin
H / 9 pICH75355
Kanamycin
H / 10 pICH79290
Ampicillin
H / 11 pICH54066
Ampicillin
Data calculated @ 2017-08-22

Kit Plate Map - #1000000044

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