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MoClo Plant Parts Kit
(Kit # 1000000047 )

Depositing Labs:   Nicola Patron

This modular cloning (MoClo) collection comprises 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation. Thirty nine parts encode promoters and 5' untranslated regions; eleven parts encode antigenic tags; eight parts encode sub-cellular localisation signals; nineteen parts encode reporter genes; six parts encode selectable marker genes; nine parts encode terminators and 3' untranslated regions. A suppressor of silencing and two linkers are also included.

This kit consists of 1 plate, and will be shipped to most countries as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

A Golden Gate Modular Cloning Toolbox for Plants. Engler C, Youles M, Gruetzner R, Ehnert T-M, Werner S, Jones JDG, Patron NJ, and Marillonnet S. ACS Synthetic Biology . 2014 Feb 20. doi: 10.1021/sb4001504. PubMed .

Description

This collection comprises 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation. Thirty nine parts encode promoters and 5' untranslated regions; eleven parts encode antigenic tags; eight parts encode sub-cellular localisation signals; nineteen parts encode reporter genes; six parts encode selectable marker genes; nine parts encode terminators and 3' untranslated regions. A suppressor of silencing and two linkers are also included.


PLoS-One-2011-MoClo-Marillonnet-Fig1.png

Image from Weber et al., PLoS One. 2011 Feb 18;6(2):e16765.

Kit Documentation

Documents included with shipment:
Icon MoClo Plant Parts Kit Info (1.2 MB)

Download a ZIP file containing all MoClo Plant Parts Kit plasmid sequences in GenBank format:
Icon MoClo Plant Parts Kit GenBank Files (247.7 KB)

Protocols

See the original ACS Synthetic Biology publication ( PubMed ) for detailed protocols for this kit.

Links

For additional information browse the [email protected] resources that have been developed by Nicola Patron and colleagues at The Sainsbury Laboratory:

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The MoClo Plant Parts Kit was a gift from Nicola Patron (Addgene kit # 1000000047)"

For your Reference section:
A Golden Gate Modular Cloning Toolbox for Plants. Engler C, Youles M, Gruetzner R, Ehnert T-M, Werner S, Jones JDG, Patron NJ, and Marillonnet S. ACS Synthetic Biology . 2014 Feb 20. doi: 10.1021/sb4001504. PubMed .

MoClo Plant Parts Kit - #1000000047

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Spectinomycin

Inventory

Well Plasmid Resistance
A / 1 pICH41373
Spectinomycin
A / 2 pICH45180
Spectinomycin
A / 3 pICH51277
Spectinomycin
A / 4 pICH45234
Spectinomycin
A / 5 pICH87611
Spectinomycin
A / 6 pICH78133
Spectinomycin
A / 7 pICSL30008
Spectinomycin
A / 8 pICSL50007
Spectinomycin
A / 9 pICSL50004
Spectinomycin
A / 10 pICH49477
Spectinomycin
A / 11 pICSL80016
Spectinomycin
A / 12 pICH44300
Spectinomycin
B / 1 pICH41388
Spectinomycin
B / 2 pICH45125
Spectinomycin
B / 3 pICH51288
Spectinomycin
B / 4 pICH45244
Spectinomycin
B / 5 pICH41402
Spectinomycin
B / 6 pICH78141
Spectinomycin
B / 7 pICSL30009
Spectinomycin
B / 8 pICSL50009
Spectinomycin
B / 9 pICSL50011
Spectinomycin
B / 10 pICSL80004
Spectinomycin
B / 11 pICSL70002
Spectinomycin
B / 12 pICH77901
Spectinomycin
C / 1 pICH45089
Spectinomycin
C / 2 pICH45131
Spectinomycin
C / 3 pICSL12006
Spectinomycin
C / 4 pICH45214
Spectinomycin
C / 5 pICH44199
Spectinomycin
C / 6 pAGM5331
Spectinomycin
C / 7 pICSL30004
Spectinomycin
C / 8 pICSL50010
Spectinomycin
C / 10 pICSL80001
Spectinomycin
C / 11 pICSL70004
Spectinomycin
C / 12 pICH77911
Spectinomycin
D / 1 pICH42211
Spectinomycin
D / 2 pICH45152
Spectinomycin
D / 3 pICH87633
Spectinomycin
D / 4 pICH87655
Spectinomycin
D / 5 pICH44222
Spectinomycin
D / 6 pAGM1482
Spectinomycin
D / 7 pICSL30006
Spectinomycin
D / 8 pICSL50012
Spectinomycin
D / 9 pICSL50006
Spectinomycin
D / 10 pICH75111
Spectinomycin
D / 11 pICSL70005
Spectinomycin
D / 12 pICH41432
Spectinomycin
E / 1 pICH50581
Spectinomycin
E / 2 pICH41551
Spectinomycin
E / 3 pICH85281
Spectinomycin
E / 4 pICH71292
Spectinomycin
E / 5 pICH44233
Spectinomycin
E / 6 pAGM5355
Spectinomycin
E / 7 pICSL30003
Spectinomycin
E / 8 pICSL50013
Spectinomycin
E / 9 pICSL80014
Spectinomycin
E / 10 pICH42222
Spectinomycin
E / 11 pICSL70008
Spectinomycin
E / 12 pICH71431
Spectinomycin
F / 1 pICH42760
Spectinomycin
F / 2 pICSL13001
Spectinomycin
F / 3 pICH88103
Spectinomycin
F / 4 pICH71301
Spectinomycin
F / 5 pICH44188
Spectinomycin
F / 6 pAGM5343
Spectinomycin
F / 7 pICSL30010
Spectinomycin
F / 8 pICSL50014
Spectinomycin
F / 9 pICH41531
Spectinomycin
F / 10 pICH43844
Spectinomycin
F / 11 pICH41414
Spectinomycin
F / 12 pICH71411
Spectinomycin
G / 1 pICH44157
Spectinomycin
G / 2 pICSL13002
Spectinomycin
G / 3 pICH87644
Spectinomycin
G / 4 pICH71311
Spectinomycin
G / 5 pICH44179
Spectinomycin
G / 6 pAGM1467
Spectinomycin
G / 7 pICH37431
Spectinomycin
G / 8 pICSL50008
Spectinomycin
G / 9 pICSL80005
Spectinomycin
G / 10 pICH44022
Spectinomycin
G / 11 pICH72400
Spectinomycin
G / 12 pICH71421
Spectinomycin
H / 1 pICH45173
Spectinomycin
H / 2 pICH51266
Spectinomycin
H / 3 pICH45195
Spectinomycin
H / 4 pICH71342
Spectinomycin
H / 5 pAGM1479
Spectinomycin
H / 6 pICSL30005
Spectinomycin
H / 7 pICH37326
Spectinomycin
H / 8 pICSL50016
Spectinomycin
H / 9 pICSL80007
Spectinomycin
H / 10 pICSL80012
Spectinomycin
H / 11 pICH41421
Spectinomycin
H / 12 pAGT707
Spectinomycin
Data calculated @ 2017-03-25

Kit Plate Map - #1000000047

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