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Plasmid-Reference_book.png Common Restriction Enzyme Sites


List of Restriction Enzymes

Below is a list of commonly used restriction enzymes. For a more complete list, visit NEB .

Nucleotide Codes

K Keto G or T
M Amino A or C
R Purine A or G
Y Pyrimidine C or T

Restriction Enzyme Sites

AatII* GACGT C MfeI C AATTG
Acc65I G GTACC MluI A CGCGT
AccI GT MKAC MscI * TGG CCA
AclI AA CGTT MspA1I CMG CKG
AatII * GACGT C MfeI C AATTG
Acc65I G GTACC MluI A CGCGT
AccI GT MKAC MscI * TGG CCA
AclI AA CGTT MspA1I CMG CKG
AfeI AGC GCT NaeI GCC GGC
AflII * C TTAAG NarI * GG CGCC
AgeI * A CCGGT NcoI * C CATGG
ApaI * GGGCC C NdeI * CA TATG
ApaLI * G TGCAC NgoMIV G CCGGC
ApoI R AATTY NheI * G CTAGC
AscI GG CGCGCC NotI * GC GGCCGC
AseI * AT TAAT NruI * TCG CGA
AsiSI GCGAT CGC NsiI ATGCA T
AvrII C CTAGG NspI RCATG Y
BamHI * G GATCC PacI TTAAT TAA
BclI * T GATCA PciI A CATGT
BglII * A GATCT PmeI GTTT AAAC
Bme1580I GKGCM C PmlI * CAC GTG
BmtI GCTAG C PsiI TTA TAA
BsaHI GR CGYC PspOMI G GGCCC
BsiEI CGRY CG PstI * CTGCA G
BsiWI C GTACG PvuI CGAT CG
BspEI T CCGGA PvuII * CAG CTG
BspHI T CATGA SacI * GAGCT C
BsrGI T GTACA SacII * CCGC GG
BssHII G CGCGC SalI * G TCGAC
BstBI * TT CGAA SbfI CCTGCA GG
BstZ17I GTA TAC ScaI AGT ACT
BtgI C CRYGG SfcI C TRYAG
ClaI * AT CGAT SfoI GGC GCC
DraI * TTT AAA SgrAI CR CCGGYG
EaeI Y GGCCR SmaI * CCC GGG
EagI * C GGCCG SmlI C TYRAG
EcoRI * G AATTC SnaBI TAC GTA
EcoRV * GAT ATC SpeI * A CTAGT
FseI GGCCGG CC SphI GCATG C
FspI * TGC GCA SspI AAT ATT
HaeII RGCGC Y StuI * AGG CCT
HincII GTY RAC SwaI ATTT AAAT
HindIII * A AGCTT XbaI * T CTAGA
HpaI * GTT AAC XhoI * C TCGAG
KasI G GCGCC XmaI C CCGGG
KpnI * GGTAC C

* Our sequence mapping program searches for these restriction sites by default. Only unique sites are displayed on the maps.

Note: Methylation can block cleavage by some restriction enzymes. In E. coli, Dam methylase affects the sequence GATC and Dcm methylase affects the sequence CCAGG or CCTGG. If these sequences are present, you will need to use a dam-, dcm- E. coli strain to grow your plasmid.