SARS-CoV-2 Spike Receptor Binding Domain Deep Mutational Scanning Library
(Pooled Library #1000000172)
This pair of duplicate libraries contains SARS-CoV-2 spike receptor binding domain (RBD) mutants in a yeast surface display vector.
Each library is barcoded with randomized N16 barcodes downstream of the coding sequence.
These libraries can be used for comprehensive mapping of the biochemical effects of RBD mutations.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||1000000172||SARS-CoV-2_RBD_DMS_Library||1||$500||Add to Cart|
Mutagenized CDSSARS-CoV-2 Receptor Binding Domain
Mutagenized region size603 bp
Total insert size1040 bp
This library will be delivered as two pooled DNA libraries in microcentrifuge tubes on blue ice (tubes labeled lib1 and lib2). The tube contents will not necessarily be frozen. For best results, minimize freeze-thaws.
For each library tube, you will receive:
The depositing laboratory provides the following GitHub repositories
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SARS-CoV-2 Spike Receptor Binding Domain Deep Mutational Scanning Library was a gift from Jesse Bloom (Addgene #1000000172)
For your References section:Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding. Starr TN, Greaney AJ, Hilton SK, Ellis D, Crawford KHD, Dingens AS, Navarro MJ, Bowen JE, Tortorici MA, Walls AC, King NP, Veesler D, Bloom JD Cell. 2020 Sep 3;182(5):1295-1310.e20. doi: 10.1016/j.cell.2020.08.012. Epub 2020 Aug 11. PubMed 32841599