SARS-CoV-2 Spike Receptor Binding Domain Deep Mutational Scanning Library

(Pooled Library #1000000172)

• Purpose:

  This pair of duplicate libraries contains SARS-CoV-2 spike receptor binding domain (RBD) mutants in a yeast surface display vector.

  Each library is barcoded with randomized N16 barcodes downstream of the coding sequence.

  These libraries can be used for comprehensive mapping of the biochemical effects of RBD mutations.

• Vector Backbone:

  pETcon_SARS-CoV-2_RBD

• Depositing Labs: Jesse Bloom
• Publication: Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. doi: 10.1016/j.cell.2020.08.012. Epub 2020 Aug 11.

Library Details

• Mutagenized CDS: SARS-CoV-2 Receptor Binding Domain
• Mutagenized region size: 603 bp
• Total insert size: 1040 bp
• Effective diversity: ~150,000

Library Shipping

This library will be delivered as two pooled DNA libraries in microcentrifuge tubes on blue ice (tubes labeled lib1 and lib2). The tube contents will not necessarily be frozen. For best results, minimize freeze-thaws.

For each library tube, you will receive:

• Volume: ∼20 µL
• Concentration: 25 ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (33 KB)
  • PCR protocol for Illumina sequencing preparation (34 KB)
• Depositor Data:
  • Table of primers (1 KB)
  • Table of codon variants (15.4 MB)
  • Plasmid map (25 KB)
• Terms and Licenses:
  • UBMTA

Depositor Comments

• Bloom Lab SARS-CoV-2 RBD DMS Library

• DOI:10.1016/j.cell.2020.08.012
  Figure S1. Available for the public to copy, distribute, or display under
  the Creative Commons Attribution 4.0 International license. (CC BY 4.0)

The depositing laboratory provides the following GitHub repositories

• SARS-CoV-2-RBD_DMS - Includes algorithms for analyzing barcode sequencing data
• SARS-CoV-2-RBD_MAP_clinical_Abs - Algorithm from Starr et al Science. 2021 (Mapping escape from antibody binding)