
SARS-CoV-2 Spike Receptor Binding Domain Deep Mutational Scanning Library
(Pooled Library #1000000172)
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Purpose
This pair of duplicate libraries contains SARS-CoV-2 spike receptor binding domain (RBD) mutants in a yeast surface display vector.
Each library is barcoded with randomized N16 barcodes downstream of the coding sequence.
These libraries can be used for comprehensive mapping of the biochemical effects of RBD mutations.
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Vector Backbone
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Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Pooled Library | 1000000172 | SARS-CoV-2_RBD_DMS_Library | 1 | $500 | Add to Cart |
Library Details
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Mutagenized CDSSARS-CoV-2 Receptor Binding Domain
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Mutagenized region size603 bp
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Total insert size1040 bp
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Effective diversity~150,000
Library Shipping
This library will be delivered as two pooled DNA libraries in microcentrifuge tubes on blue ice (tubes labeled lib1 and lib2). The tube contents will not necessarily be frozen. For best results, minimize freeze-thaws.
For each library tube, you will receive:
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Volume∼20 µL
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Concentration25 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Depositor Comments

Bloom Lab SARS-CoV-2 RBD DMS Library
- DOI:10.1016/j.cell.2020.08.012
Figure S1. Available for the public to copy, distribute, or display under
the Creative Commons Attribution 4.0 International license. (CC BY 4.0)
The depositing laboratory provides the following GitHub repositories
- SARS-CoV-2-RBD_DMS - Includes algorithms for analyzing barcode sequencing data
- SARS-CoV-2-RBD_MAP_clinical_Abs - Algorithm from Starr et al Science. 2021 (Mapping escape from antibody binding)
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SARS-CoV-2 Spike Receptor Binding Domain Deep Mutational Scanning Library was a gift from Jesse Bloom (Addgene #1000000172) -
For your References section:
Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding. Starr TN, Greaney AJ, Hilton SK, Ellis D, Crawford KHD, Dingens AS, Navarro MJ, Bowen JE, Tortorici MA, Walls AC, King NP, Veesler D, Bloom JD Cell. 2020 Sep 3;182(5):1295-1310.e20. doi: 10.1016/j.cell.2020.08.012. Epub 2020 Aug 11. PubMed 32841599