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pETcon_SARS-CoV-2_RBD
(Plasmid #166782)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 166782 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pETcon(-) (Plasmid #41522)
  • Backbone manufacturer
    Andrew Scharenberg
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spike receptor binding domain (RBD) from SARS-CoV-2
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETcon_SARS-CoV-2_RBD was a gift from Jesse Bloom (Addgene plasmid # 166782 ; http://n2t.net/addgene:166782 ; RRID:Addgene_166782)
  • For your References section:

    Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding. Starr TN, Greaney AJ, Hilton SK, Ellis D, Crawford KHD, Dingens AS, Navarro MJ, Bowen JE, Tortorici MA, Walls AC, King NP, Veesler D, Bloom JD. Cell. 2020 Sep 3;182(5):1295-1310.e20. doi: 10.1016/j.cell.2020.08.012. Epub 2020 Aug 11. 10.1016/j.cell.2020.08.012 PubMed 32841599