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Monosiga cDNA library
(Pooled Library #25864)

  • Purpose

    cDNA library for Monosiga brevicollis

  • Vector Backbone


Item Catalog # Description Quantity Price (USD)
Pooled Library 25864
  • cDNA library

Available to Academic and Nonprofits Only

Library Details

  • Species
    Monosiga brevicollis

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼100 µL
  • Concentration
    100 ng/µL

Resource Information

Depositor Comments

cDNA Library Construction. Double-stranded cDNA was synthesized with SuperScriptII RT and DNA polymerase I and ligated to adapters containing a SalI restriction site (GIBCO/BRL). To remove residual adapters and enrich for full-length fragments, cDNA was first size-fractionated by column chromatography and then ligated to predigested pSportI vector (GIBCO/BRL). Transformation of DH5α with the ligated DNA yielded a plasmid library with approximately 8.8 x 10 4 colony-forming units.

Note: Addgene has sequenced part of this library. See the Addgene Data link above for more information.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    Monosiga cDNA library was a gift from Sean Carroll & Nicole King (Addgene # 25864)
  • For your References section:

    A receptor tyrosine kinase from choanoflagellates: molecular insights into early animal evolution . King N, Carroll SB. Proc Natl Acad Sci U S A. 2001 Dec 18. 98(26):15032-7. 10.1073/pnas.261477698PubMed 11752452