Weissman Lab Target Site Library (PCT48)
(Pooled Library #170392)
This lentiviral library includes a constitutively expressed GFP cassette with a lineage tracing "Target Site". The 3' UTR of GFP contains three Cas9-targetable spacer sequences and a random 14-bp barcode at high sequence diversity.
The three cut-sites in the Target Site are complementary to the three sgRNAs expressed from the triple-sgRNA-BFP-Puromycin construct, Plasmid 170391: PCT62.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||170392||PCT48||1||$600||Add to Cart|
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Target Site Library (PCT48) was a gift from Jonathan Weissman (Addgene #170392)
For your References section:Inference of single-cell phylogenies from lineage tracing data using Cassiopeia. Jones MG, Khodaverdian A, Quinn JJ, Chan MM, Hussmann JA, Wang R, Xu C, Weissman JS, Yosef N. Genome Biol. 2020 Apr 14;21(1):92. doi: 10.1186/s13059-020-02000-8. PubMed 32290857Single-cell lineages reveal the rates, routes, and drivers of metastasis in cancer xenografts. Quinn JJ, Jones MG, Okimoto RA, Nanjo S, Chan MM, Yosef N, Bivona TG, Weissman JS. Science. 2021 Feb 26;371(6532). pii: science.abc1944. doi: 10.1126/science.abc1944. Epub 2021 Jan 21. PubMed 33479121