Genome-wide Mouse Lentiviral CRISPR gRNA Library v1
(Pooled Library #50947)
Note: This library has been replaced by an updated version (see below).
A gRNA library against 19,150 mouse protein coding genes, designed for use in lentivirus.
- Vector Backbone
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP- does not express Cas9
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Genome-wide mouse lentiviral CRISPR gRNA library was a gift from Kosuke Yusa (Addgene #50947)
For your References section:Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library . Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K. Nat Biotechnol. 2014 Mar;32(3):267-73. doi: 10.1038/nbt.2800. Epub 2013 Dec 23. 10.1038/nbt.2800PubMed 24535568