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452 Results for:  D10

Your Selections:

Plasmids, Strains, and Pooled Libraries 1–20 of 452

  1. Plasmid
    #58695
    Depositor
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein Human
    Use
    AAV, CRISPR
    Tags
    NLS, HA Tag, GyrA Nsplit Intein
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutat...
  2. Plasmid
    #12473
    Depositor
    Insert
    Top7 D9T D10V N14T
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    D9T D10V N14T
  3. Plasmid
    #42333
    Depositor
    Purpose
    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
  4. Plasmid
    #113022
    Depositor
    Purpose
    Expresses GCN4-D10A in mammalian cells
    Insert
    GCN4-D10A ( GCN4 Budding Yeast; S. pyogenes )
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    D10A
  5. Plasmid
    #42335
    Depositor
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
  6. Plasmid
    #112134
    Depositor
    Purpose
    lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)
    Insert
    D10Acas9-3xflag-blast Synthetic
    Use
    Lentiviral, CRISPR
    Tags
    None
    Expression
    Mammalian
    Mutation
    D10A mutant in Cas9
  7. Plasmid
    #113025
    Depositor
    Purpose
    Expresses D10A-GCN4 in mammalian cells
    Insert
    D10A-GCN4 ( GCN4 Budding Yeast; S. pyogenes )
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    D10A
  8. Plasmid
    #74495
    Depositor
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Insert
    Cas9 D10A Synthetic
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    D10A
  9. Plasmid
    #58772
    Depositor
    Purpose
    Expresses Cas9 nickase and gRNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase S. pyogenes
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
  10. Plasmid
    #59984
    Depositor
    Purpose
    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette
    Insert
    Cas9-D10A Synthetic
    Use
    CRISPR
    Tags
    HA
    Expression
    Insect
    Mutation
    D10A
  11. Plasmid
    #58777
    Depositor
    Purpose
    Expresses Cas9 nickase and gRNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase S. pyogenes
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
  12. Plasmid
    #61477
    Depositor
    Purpose
    based on pDe-CAS9, encodes Cas9 D10A nickase variant
    Insert
    Cas9-D10A Mustard Weed
    Use
    CRISPR
    Tags
    None
    Expression
    Plant
    Mutation
    catalytic Asp10 changed to Ala
  13. Plasmid
    #60815
    Depositor
    Purpose
    dCas9 with single cysteine residue (M1C) for site-specific labelling
    Insert
    dCas9 M1C D10A C80S H840A C574S S. pyogenes
    Use
    Tags
    MBP, His10
    Expression
    Bacterial
    Mutation
    M1C D10A C80S H840A C574S
  14. Plasmid
    #58776
    Depositor
    Purpose
    Expresses Cas9 nickase and gRNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase S. pyogenes
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
  15. Plasmid
    #12474
    Depositor
    Insert
    Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    b19 only. N7K D9T D10V N14T F...
  16. Plasmid
    #58774
    Depositor
    Purpose
    Expresses Cas9 nickase and gRNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase S. pyogenes
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
  17. Plasmid
    #58775
    Depositor
    Purpose
    Expresses Cas9 nickase and gRNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase S. pyogenes
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
  18. Plasmid
    #74618
    Depositor
    Purpose
    Expresses a double mutant truncation of Hook3 in bacteria for purification
    Insert
    human Hook3 amino acids 1-239, D102A, E108A ( HOOK3 Human )
    Use
    Tags
    6x His, Strep II, superfolder...
    Expression
    Bacterial
    Mutation
    D102A, E108A
  19. Plasmid
    #60367
    Depositor
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Insert
    RNase HI-D10R-E48R Escherichia coli
    Use
    Tags
    mCherry, NLS from SV40 T antigen
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
  20. Plasmid
    #58773
    Depositor
    Purpose
    Expresses Cas9 nickase and gRNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase S. pyogenes
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
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