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Addgene
Showing: 1 - 20 of 1805 results

  9. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    44720-plasmid-map-sequence-id-179979

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  10. hCas9_D10A

    Plasmid
    #41816
    Purpose
    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Jan 3.
    Insert
    Cas9_D10A
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A nickase
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    41816-plasmid-map-sequence-id-116715

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  13. pPD107.09

    Plasmid
    #1703
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SupTrp5', SupTrp3', T7p, lacZN, OriF1&g…
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    1703-plasmid-map-sequence-id-309

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  14. pPD10_46

    Plasmid
    #1432
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    OriF1<<, OriF1>>, lacZN, unc54N, unc5…
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    1432-plasmid-map-sequence-id-43

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  15. pABD1031

    Plasmid
    #68004
    Purpose
    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    Use
    Tags
    eYFP
    Expression
    Plant
    Mutation
    Promoter
    2X Cauliflower Mosaic Virus 35S promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 1805 results