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4 Results for:  amilCP

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Plasmids, Strains, and Pooled Libraries 1–4 of 4

  1. Plasmid
    #85166
    Depositor
    Purpose
    For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.
    Insert
    EcoRI Methylase-AmilCP E. coli strain RY13
    Use
    Tags
    AmilCP
    Expression
    Bacterial
    Mutation
    None
  2. Plasmid
    #85165
    Depositor
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences E. coli strain RY13
    Use
    Tags
    AmilCP, RTX
    Expression
    Bacterial
    Mutation
    None
  3. Plasmid
    #102680
    Depositor
    Purpose
    Mobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable parts
    Insert
    J23106:B0034:amilCP:rrnBT1-T7TE
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Mutation
    None
  4. PGR-Blue Empty backbone
    Plasmid
    #68374
    Depositor
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Use
    Synthetic Biology ; Golden Ga...
    Tags
    None
    Expression
    Bacterial
    Mutation
    None