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4 Results for: amilCP
Plasmids, Strains, and Pooled Libraries 1–4 of 4
Plasmid#85166DepositorPurposeFor methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.InsertEcoRI Methylase-AmilCP E. coli strain RY13UseTagsAmilCPExpressionBacterialMutationNone
Plasmid#85165DepositorPurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.InsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences E. coli strain RY13UseTagsAmilCP, RTXExpressionBacterialMutationNone
Plasmid#102680DepositorPurposeMobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable partsInsertJ23106:B0034:amilCP:rrnBT1-T7TEUseSynthetic BiologyTagsNoneExpressionMutationNone
PGR-Blue Empty backbonePlasmid#68374DepositorArticlePurposeTerminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.UseSynthetic Biology ; Golden Ga...TagsNoneExpressionBacterialMutationNone