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We narrowed to 3,743 results for: golden gate

Showing: 181 - 200 of 3743 results

  1. pOPS0253

    Plasmid
    #115505
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115505-plasmid-map-sequence-id-224288

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  3. pGGD_MiniTurboID-YFP

    Plasmid
    #222434
    Purpose
    Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.
    Depositor
    Dominique Bergmann
    Article
    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    222434-plasmid-map-sequence-id-440831

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  4. pGGB_YFP-MiniTurboID

    Plasmid
    #222433
    Purpose
    Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.
    Depositor
    Dominique Bergmann
    Article
    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    222433-plasmid-map-sequence-id-440826

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  5. pGGG-TaBSS1-tdTom

    Plasmid
    #242014
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Use
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    BSS1
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242014-plasmid-map-sequence-id-487879

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  6. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  7. pDM068

    Plasmid
    #216811
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216811-plasmid-map-sequence-id-430142

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  8. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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  9. pZA8

    Plasmid
    #158486
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, D481V, STOP codon
    Promoter
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158486-plasmid-map-sequence-id-310749

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  11. pNDGG008

    Plasmid
    #231312
    Purpose
    Vector part for BEVA; BEVA2.0 Position 4 sacB sucrose counterselection module, AmpR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 4 sacB sucrose counterselection module, AmpR.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231312-plasmid-map-sequence-id-462975

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  12. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

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  13. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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  14. pZA7

    Plasmid
    #158485
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, STOP codon
    Promoter
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158485-plasmid-map-sequence-id-310754

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  15. pZA15

    Plasmid
    #158493
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, no STOP codon
    Promoter
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158493-plasmid-map-sequence-id-310753

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  16. pZA6

    Plasmid
    #158484
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D481V, STOP codon
    Promoter
    Available Since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158484-plasmid-map-sequence-id-309731

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  18. pZA14

    Plasmid
    #158492
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D481V, no STOP codon
    Promoter
    Available Since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158492-plasmid-map-sequence-id-309703

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  19. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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  20. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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Showing: 181 - 200 of 3743 results