Skip to main content
Addgene
Showing: 181 - 200 of 3070 results
  1. pKEK3199

    Plasmid
    #234097
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Insert
    tspurple
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. pKEK3200

    Plasmid
    #234098
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Insert
    fuGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pKEK3229

    Plasmid
    #234101
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Insert
    eforCP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pKEK3232

    Plasmid
    #234104
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Insert
    tsPurple
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pKEK3233

    Plasmid
    #234105
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Insert
    YukonOFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pKEK3395

    Plasmid
    #234079
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Insert
    YukonOFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pKEK3398

    Plasmid
    #234082
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Insert
    sfCherry2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pKEK3193

    Plasmid
    #234093
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Insert
    eforCP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. L0I80_Bxb1mut(noBsaI)_GG1-GG4

    Plasmid
    #219715
    Purpose
    Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.
    Depositor
    Insert
    GG1-Bxb1(noBsaI0)-GG4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pOPS0254

    Plasmid
    #115506
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB
    Depositor
    Insert
    Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pOPS0377

    Plasmid
    #115509
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP
    Depositor
    Insert
    sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pOPS0379

    Plasmid
    #115510
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP
    Depositor
    Insert
    Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pOPS0750

    Plasmid
    #115511
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.
    Depositor
    Insert
    gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  14. pOPS0314

    Plasmid
    #115507
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB
    Depositor
    Insert
    celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. pOPS0253

    Plasmid
    #115505
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.
    Depositor
    Insert
    Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. pGGD_MiniTurboID-YFP

    Plasmid
    #222434
    Purpose
    Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.
    Depositor
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. pGGB_YFP-MiniTurboID

    Plasmid
    #222433
    Purpose
    Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.
    Depositor
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 181 - 200 of 3070 results