We narrowed to 3,913 results for: golden gate

1. pEPOR0SP0020

   Plasmid

   #117531

   Purpose

   Level0 Golden Gate part: CDS, SaCas9

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SaCas9 (with stop codon)
   

   Use

   CRISPR

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH183

   Plasmid

   #122856

   Purpose

   MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences

   Depositor

   Markus Schmid

   Article

   Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

   Insert

   MS2-modified sgRNA targeting the Arabidopsis FT promoter
   

   Use

   Golden gate compatible cloning vector

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPD151 ZF37i

   Plasmid

   #138778

   Purpose

   Constitutive expression of ZF37i under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF37i
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas12c-sgRNA_BbsI_CAM

   Plasmid

   #183221

   Purpose

   Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c single guide RNA containing BbsI sites in spacer sequence
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBlue-TAL

   Plasmid

   #49401

   Purpose

   The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

   Depositor

   Michal Zurovec

   Article

   Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

   Type

   Empty backbone

   Use

   TALEN

   Expression

   Insect

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCBLsgRNA3

   Plasmid

   #199729

   Purpose

   Golden Gate entry vector; 3rd gRNA under TaU3 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   TaU3-sgRNA3 scaffold
   

   Expression

   Plant

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBLsgRNA4

   Plasmid

   #199730

   Purpose

   Golden Gate entry vector; 4th gRNA under ZmU6-C1 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   ZmU6C1-sgRNA4 scaffold
   

   Expression

   Plant

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBLsgRNA5

   Plasmid

   #199731

   Purpose

   Golden Gate entry vector; 5th gRNA under ZmU6-C3 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   ZmU6C3-sgRNA5 scaffold
   

   Expression

   Plant

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCBLsgRNA6

   Plasmid

   #199732

   Purpose

   Golden Gate entry vector; 6th gRNA under OsU6.1 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   OsU6.1-sgRNA6 scaffold
   

   Expression

   Plant

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCBLsgRNA7

    Plasmid

    #199733

    Purpose

    Golden Gate entry vector; 7th gRNA under OsU6.2 promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    OsU6.2-sgRNA7 scaffold
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBLsgRNA8

    Plasmid

    #199734

    Purpose

    Golden Gate entry vector; 8th gRNA under TaU6 promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    TaU6-sgRNA8 scaffold
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. BCJJ339

    Plasmid

    #117501

    Purpose

    RPS5a:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtRPS5a

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPOR0SP0013

    Plasmid

    #117521

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (with stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ147

    Plasmid

    #69299

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGGF002

    Plasmid

    #48843

    Purpose

    Provides a BASTA resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    p35S::BastaR::t35S
    

    Use

    Golden gate compatible cloning vector

    Mutation

    internal BsaI recognition site in 35S promoter re…

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. BCJJ358

    Plasmid

    #117505

    Purpose

    RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtRPS5a

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. BCJJ344

    Plasmid

    #117503

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtUBI10

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNM3749

    Plasmid

    #154108

    Purpose

    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    

    Use

    Cre/Lox; Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. L0-I18 Ub-Arg GG1-GG2

    Plasmid

    #195899

    Purpose

    Level 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    Ub-Arg tag
    

    Use

    Synthetic Biology

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPD355 VPR-FRB (NLS)

    Plasmid

    #138847

    Purpose

    Constitutive expression of VPR-FRB under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-FRB
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge