We narrowed to 2,934 results for: D10

1. pJMP1335

   Plasmid

   #119269

   Purpose

   rfp "test" strain

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac (GST-preSci site-CD45)

   Plasmid

   #177889

   Purpose

   Baculo expression of GST tag fused to human CD45 ICD

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   CD45 (aa 598-1304 only) (PTPRC Human)
   

   Use

   Tags

   TEV-GST-PreScission cut site

   Expression

   Insect

   Mutation

   L652P

   Promoter

   Polyhedrin

   Available since

   Aug. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUPER.retro-{beta}4 siRNA

   Plasmid

   #16266

   Depositor

   Arthur Mercurio

   Article

   Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

   Insert

   SIRNA Beta 4 Integrin (ITGB4 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1-CibN-dCas9

   Plasmid

   #60551

   Purpose

   Expresses CibN-dCas9 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   CibN-dCas9
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEL033

   Plasmid

   #137902

   Purpose

   STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AYBEv0.1

   Plasmid

   #193966

   Purpose

   A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   ecTadA8e-nSpCas9(D10A)-MPG(wt)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a, CBh

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. NAX_CAGGS_CAS9_m4_KRAB_KanR neo

   Plasmid

   #167877

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FH-TET3-HxDmut-pEF

   Plasmid

   #127896

   Purpose

   Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A

   Depositor

   Patrick Hogan, Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   TET3-HxDmut (TET3 Human)
   

   Use

   Tags

   HA and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXLE-dCas9VPH-T2A-GFP-shP53

   Plasmid

   #102895

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPH-T2A-GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJMP1363

    Plasmid

    #119279

    Purpose

    for stabilizing ICE in the presence of rapI expression

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJMP1171

    Plasmid

    #119253

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJMP1170

    Plasmid

    #119252

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-dCas9-CibN

    Plasmid

    #60552

    Purpose

    Expresses dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXPR_003 sgNFkB2 guide 2

    Plasmid

    #193594

    Purpose

    NFkB2 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgNFkB2 guide 2 (NFKB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NAX_CAGGS_CAS9_m4_KanR neo

    Plasmid

    #167869

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NAX_CAGGS_CAS9_m4_VPR_Hygro

    Plasmid

    #167883

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-mFzd2-1D4

    Plasmid

    #42264

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 2 (Fzd2 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pJMP1217

    Plasmid

    #119258

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330-HA-dSpCas9

    Plasmid

    #92112

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9
    

    Use

    CRISPR

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. A3A-PBE-ΔUGI

    Plasmid

    #119770

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-ΔUGI-NLS
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge