We narrowed to 16,257 results for: form

1. pEGFPC1/GgVcl 1-258 (aka VD1)

   Plasmid

   #46270

   Depositor

   Susan Craig

   Article

   Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

   Insert

   Vcl 1-258 (aka VD1) (VCL Chicken)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   1-258 (VD1 domain)

   Promoter

   CMV

   Available Since

   July 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPEE17

   Plasmid

   #128361

   Purpose

   Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.

   Depositor

   James Fraser

   Article

   Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

   Insert

   Acetamidase (amdS)
   

   Use

   Unspecified

   Promoter

   TEF1

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cherry-Myo1b

   Plasmid

   #187366

   Purpose

   Expresses rat myosin 1b isoform b domain labelled with Cherry

   Depositor

   Evelyne Coudrier

   Article

   Iuliano et al J Cell Biol. 2018 Jun 4;217(6):2033-2046

   Insert

   myosin 1b isoform b (Myo1b Rat)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET SUMO-FL-syt1

   Plasmid

   #213628

   Purpose

   Bacterial expression of SUMO-tagged rat FL-syt1

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt1 (Syt1 Rat)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEBB-II-I-deltaNLS1-EGFP

   Plasmid

   #22150

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 297-304

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHYDuet93

   Plasmid

   #42303

   Depositor

   Hideo Iwai

   Article

   Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

   Insert

   NpuDnaEdC6
   

   Tags

   H6-GB1

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEBG-TFII-deltaNLS1

   Plasmid

   #22153

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   deletion of amino acids 297-304

   Available Since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET SUMO-FL-syt7

   Plasmid

   #213630

   Purpose

   Bacterial expression of SUMO-tagged mouse FL-syt7

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt7 (Syt7 Mouse)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHYBAD94

   Plasmid

   #42305

   Depositor

   Hideo Iwai

   Article

   Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

   Insert

   NpuDnaEC6
   

   Tags

   GB1-H6

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b/GgVcl 1-258 A50I mutation

    Plasmid

    #46175

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-258 A50I (VCL Chicken)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-258 (VD1) A50I mutation; talin binding defic…

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pACEC3GH (Hyg)

    Plasmid

    #110084

    Purpose

    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLAG_attP

    Plasmid

    #110095

    Purpose

    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3xFLAG

    Expression

    Bacterial

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET26b_Nb.b201

    Plasmid

    #131404

    Purpose

    E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody b201
    

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCHC042 (∆phaCAB)

    Plasmid

    #226208

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for phaCAB
    

    Use

    Synthetic Biology

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCHC005 (∆RE)

    Plasmid

    #226199

    Purpose

    Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for type I restriction-modification system restriction subunit H16_A0006
    

    Use

    Synthetic Biology

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pESUMO-hRORg1

    Plasmid

    #170330

    Purpose

    Expresses RORg isoform 1 with N-terminal HisSUMO tag in bacteria

    Depositor

    Patrick Griffin

    Article

    Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

    Insert

    RORG isoform 1 (RORC Human)
    

    Tags

    HisSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCHC039 (∆phcA)

    Plasmid

    #226207

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for PhcA
    

    Use

    Synthetic Biology

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. GST-beta-arrestin1

    Plasmid

    #36918

    Depositor

    Robert Lefkowitz

    Article

    Nobles et al J Biol Chem. 2007 Jul 20;282(29):21370-8

    Insert

    beta-arrestin1 (Arrb1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pACEC3GH (Apr)

    Plasmid

    #110085

    Purpose

    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFPC1/GgVcl 1-258 A50I mutation

    Plasmid

    #46271

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-258 (aka VD1) A50I mutation (VCL Chicken)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    1-258 (VD1 domain) A50I mutation

    Promoter

    CMV

    Available Since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge