We narrowed to 3,913 results for: golden gate

1. L0-I39 PhiC31-Intein GG1-GG4

   Plasmid

   #195905

   Purpose

   Level 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   PhiC31-intein
   

   Use

   Synthetic Biology

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. L0-I40 Intein-PhiC31 GG1-GG4

   Plasmid

   #195906

   Purpose

   Level 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   Intein-PhiC31
   

   Use

   Synthetic Biology

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BCJJ340

   Plasmid

   #117502

   Purpose

   UBI10:Cas9:E9 Golden Gate Level 1 Position 2

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtUBI10

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGGF008

   Plasmid

   #48848

   Purpose

   Provides a BASTA resistance cassette as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   pNOS::BastaR::tNOS
   

   Use

   Golden gate compatible cloning vector

   Mutation

   chi sequence in BastaR removed by silent substitu…

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLY134

   Plasmid

   #130962

   Purpose

   Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB5
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Anderson promoter J23100

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEPOR1CB0058

   Plasmid

   #117539

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   sgRNA (SaCas9)
   

   Expression

   Plant

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZA55

   Plasmid

   #174768

   Purpose

   Entry clone for Golden Gate assembly

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-L17E without STOP codon
   

   Use

   Unspecified

   Mutation

   L17E

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZA56

   Plasmid

   #174769

   Purpose

   Entry clone for Golden Gate assembly

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-L17E/D481V without STOP codon
   

   Use

   Unspecified

   Mutation

   L17E/D481V

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZA54

   Plasmid

   #174767

   Purpose

   Entry clone for Golden Gate assembly

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-D481V without STOP codon
   

   Use

   Unspecified

   Mutation

   D481V

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZA53

    Plasmid

    #174766

    Purpose

    Entry clone for Golden Gate assembly

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn without STOP codon
    

    Use

    Unspecified

    Mutation

    WT

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

    Plasmid

    #162810

    Purpose

    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    BsmBI_Intein_DD
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMH184

    Plasmid

    #122857

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPOR0SP0007

    Plasmid

    #117534

    Purpose

    Level0 Golden Gate part: CDS, LbCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    LbCas12a (with stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGGD007

    Plasmid

    #48837

    Purpose

    Provides linker:nuclear localization signal as GreenGate C-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    linker:nuclear localization signal
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPOR0SP0021

    Plasmid

    #117532

    Purpose

    Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (no stop codon)
    

    Use

    CRISPR

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPOR0SP0016

    Plasmid

    #117526

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.0

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.0 (with stop codon)
    

    Use

    CRISPR

    Mutation

    K810A, K1003A, R1060A

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EML6442_p35S-mCherry2-SV40

    Plasmid

    #234351

    Purpose

    Expressed mCherry2 florescent proteins in plant cells for tracing transformation events

    Depositor

    Erh-Min Lai

    Article

    Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

    Insert

    SpeR
    

    Expression

    Plant

    Available Since

    Nov. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DHFR-TEV (integrating)

    Plasmid

    #247218

    Purpose

    Plasmid for genomic integration of DHFR-TEV into the YORWΔ22 locus of yeast cells.

    Depositor

    Johannes Herrmann

    Article

    Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

    Insert

    DHFR-TEV
    

    Expression

    Yeast

    Promoter

    TEF2

    Available Since

    Nov. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VN1272 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-2GG+rpoA-IP3

    Plasmid

    #235120

    Purpose

    Quantitative bacterial two-hybrid (qB2H). Expresses IP3.

    Depositor

    Oscar Ramos

    Article

    Guyot et al bioRxiv 2025.10.29.680610

    Type

    Empty backbone

    Use

    Synthetic Biology; Quantitative bacterial two-hyb…

    Tags

    rpoA

    Expression

    Bacterial

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L3928 Filler DNA

    Plasmid

    #244193

    Purpose

    Empty vector; modified pcDNA with CMV promoter and bGHpolyA removed to reduce burden

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge