We narrowed to 3,792 results for: golden gate

1. pMH184

   Plasmid

   #122857

   Purpose

   MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

   Depositor

   Markus Schmid

   Article

   Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

   Insert

   MS2-modified sgRNA targeting the Arabidopsis FT promoter
   

   Use

   Golden gate compatible cloning vector

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPOR0SP0007

   Plasmid

   #117534

   Purpose

   Level0 Golden Gate part: CDS, LbCas12a

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   LbCas12a (with stop codon)
   

   Use

   CRISPR

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGD007

   Plasmid

   #48837

   Purpose

   Provides linker:nuclear localization signal as GreenGate C-terminal tag module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   linker:nuclear localization signal
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPOR0SP0021

   Plasmid

   #117532

   Purpose

   Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SaCas9 (no stop codon)
   

   Use

   CRISPR

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPOR0SP0016

   Plasmid

   #117526

   Purpose

   Level0 Golden Gate part: CDS, Sp-eCas9 1.0

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-eCas9 1.0 (with stop codon)
   

   Use

   CRISPR

   Mutation

   K810A, K1003A, R1060A

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. L3928 Filler DNA

   Plasmid

   #244193

   Purpose

   Empty vector; modified pcDNA with CMV promoter and bGHpolyA removed to reduce burden

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. p0AmpCD

   Plasmid

   #237276

   Purpose

   Empty vector for L0 TypeIIS

   Depositor

   Eric Young

   Article

   Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p0AmpAB

   Plasmid

   #237274

   Purpose

   Empty vector for L0 TypeIIS

   Depositor

   Eric Young

   Article

   Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tad-CBE2.1

   Plasmid

   #235053

   Purpose

   Expresses Tad-CBE2.1 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE2.1-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tad-CABE2.5

    Plasmid

    #235054

    Purpose

    Expresses Tad-CABE2.5 in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CABE2.5-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tad-CBE3.1

    Plasmid

    #235056

    Purpose

    Expresses Tad-CBE3.1 in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE3.1-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tad-CBE3.2

    Plasmid

    #235057

    Purpose

    Expresses Tad-CBE3.2 in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE3.2-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tad-CABE1.1

    Plasmid

    #235051

    Purpose

    Expresses Tad-CABE1.1 in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CABE1.1-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Tad-CABE1.2

    Plasmid

    #235052

    Purpose

    Expresses Tad-CABE1.2 in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CABE1.2-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTE5425

    Plasmid

    #229032

    Purpose

    Expression vector for 10xHis-phi29 DNA polymerase (catalytically inactive mutant D249E): PT7-lacO-g10leader-p2 dead-T7 terminator, CamR

    Depositor

    Tobias Erb

    Article

    Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

    Insert

    PT7-lacO-g10lead-10xHisTag-phi29 DNAP (D249E, catalytically inactive)
    

    Tags

    10x His-tag + Factor Xa recognition site

    Expression

    Bacterial

    Promoter

    PT7-lacO and T7 promoter

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSWITCH

    Plasmid

    #222402

    Purpose

    Expression in Streptococcus pyogenes

    Depositor

    Anne Botteaux

    Article

    Schiavolin et al Microbiol Spectr. 2024 Oct 3;12(10):e011

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. Hv_HPT:pCSRM2C:Gus_introns

    Plasmid

    #215191

    Purpose

    Evaluating guard cell specific promotors in Barley

    Depositor

    Penny Hundleby

    Article

    Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

    Insert

    Scream2
    

    Promoter

    BdSCRM2

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bo_NPTII:pCSRM2C:Gus_introns

    Plasmid

    #215177

    Purpose

    Evaluating guard cell specific promotors in Brassica

    Depositor

    Penny Hundleby

    Article

    Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

    Insert

    Scream2
    

    Use

    Synthetic Biology

    Promoter

    pSCRM2

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRO288

    Plasmid

    #233827

    Purpose

    MoClo-YTK; type4A; Activation domains; VP48

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    VP48
    

    Expression

    Yeast

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRO129

    Plasmid

    #233805

    Purpose

    MoClo-YTK; type3; rtTA systems; rtTA3

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    rtTA3
    

    Expression

    Yeast

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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