We narrowed to 4,154 results for: pCMV

1. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

   Plasmid

   #176458

   Purpose

   Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-ND1 Right-GSVG-UGI

   Plasmid

   #187413

   Purpose

   human mitochondrial DNA ND1 target TALE

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA GSVG variant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

   Plasmid

   #99229

   Purpose

   Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos2
   

   Use

   Tags

   Lifeact

   Expression

   Mammalian

   Mutation

   A69T for primed conversion applications

   Promoter

   CMV

   Available since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcmv3-STING-C64S,C65S-FLAG

   Plasmid

   #198186

   Purpose

   Mammalian expression of FLAG-tagged mouse STING1 C64S, C65S

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   TMEM173 (Sting1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The cysteine in STING at position 64/65 mutates t…

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

   Plasmid

   #74261

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   p.Y591X truncation

   Promoter

   CMV

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pCMV6-G1-ATP8-Myc-FLAG (G418)

   Plasmid

   #86850

   Purpose

   mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

   Depositor

   Matthew O'Connor

   Article

   Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

   Insert

   ATP8 (ATP8 Human)
   

   Use

   Tags

   ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV/myc/Nuc-mutant-hogg1

   Plasmid

   #18710

   Depositor

   David Sidransky

   Article

   Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

   Insert

   8-oxoguanine DNA glycosylase (OGG1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino Acid 229 Arginine changed to Glutamine

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-Flag-YAP-S94/F95A

   Plasmid

   #33057

   Depositor

   Kunliang Guan

   Article

   Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S94A, F95A

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

   Plasmid

   #163545

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Polymerase expression - pCMV-ePhi29 (LM2802)

    Plasmid

    #208967

    Purpose

    Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

    Plasmid

    #178868

    Purpose

    Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV5 MYC-Smurf2 (FF29/30A) WT

    Plasmid

    #24604

    Depositor

    Jeff Wrana

    Article

    Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

    Insert

    Smurf2 (SMURF2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    F29A, F30A

    Promoter

    Available since

    April 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. 3XFlag pCMV5-TOPO TAZ (∂393)

    Plasmid

    #24812

    Depositor

    Jeff Wrana

    Article

    Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Contains ONLY amino acids 1-393. (No PDZ binding…

    Promoter

    Available since

    June 3, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid

    #208965

    Purpose

    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-FLAG TIF1gamma M (456-887)

    Plasmid

    #15735

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    TIF1 gamma M (TRIM33 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Middle region (amino acids 456-887).

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid

    #174140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV Gab1(PRD)-mCherry-HOTag3

    Plasmid

    #190026

    Purpose

    Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

    Depositor

    Lukasz Bugaj

    Article

    Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

    Insert

    Gab1(PRD) CluMPS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV Flag dPDZ-PTEN (1-400)

    Plasmid

    #22232

    Depositor

    Hong Wu

    Article

    Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

    Insert

    PTEN (Pten Mouse)
    

    Use

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Mutation

    PDZ binding domain deleted. Contains amino acids …

    Promoter

    Available since

    March 19, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-Flag-YAP-4SA(S109)

    Plasmid

    #33081

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S61A, S127A, S164A, S381A

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-Flag-YAP-S163/164A

    Plasmid

    #33089

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S163A, S164A

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only