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We narrowed to 3,461 results for: zebrafish

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Showing: 2021 - 2040 of 3461 results

  6. PDI-QUAD-V5-pcDNA3.1

    Plasmid
    #153550
    Purpose
    PDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.
    Depositor
    Julie Atkin
    Publication
    Parakh et al iScience. 2020 May 22;23(5):101097. doi:
    Insert
    Protein disulphide isomerase
    Tags
    V5 tag
    Mutation
    C53S, C56S, C397S, C400S
    Promoter
    pCMV
    Available since
    Aug. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    153550-plasmid-map-sequence-id-308584

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  7. pCSCRET2(#323)

    Plasmid
    #184058
    Purpose
    cyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Publication
    Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1
    Insert
    6myc-CRE-ERT2
    Use
    Synthetic Biology
    Tags
    6xMyc
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184058-plasmid-map-sequence-id-365949

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  11. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Expression
    Bacterial
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79889-plasmid-map-sequence-id-150065

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  14. pT2iU6m5Hoxa13bE4PCh(#765)

    Plasmid
    #184102
    Purpose
    cyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-ZfHoxa13b-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes) + in vitro tra…
    Tags
    5xMyc
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Ubi+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184102-plasmid-map-sequence-id-365950

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  20. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80061
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80061-plasmid-map-sequence-id-152595

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Showing: 2021 - 2040 of 3461 results