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In vivo Validation of Bimolecular Fluorescence Complementation (BiFC) to Investigate Aggregate Formation in Amyotrophic Lateral Sclerosis (ALS).
Don EK, Maschirow A, Radford RAW, Scherer NM, Vidal-Itriago A, Hogan A, Maurel C, Formella I, Stoddart JJ, Hall TE, Lee A, Shi B, Cole NJ, Laird AS, Badrock AP, Chung RS, Morsch M
Mol Neurobiol. 2021 May;58(5):2061-2074. doi: 10.1007/s12035-020-02238-0. Epub 2021 Jan 7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
162608pCS2plus-TDP-43Allows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assays
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162609pCS2plus-TDP-43 M337VAllows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assays
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162610pCS2plus-mVenus1-155-GGGSAllows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays
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162611pCS2plus-mVenus1-155-GGGS-TDP-43Allows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays
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162612pCS2plus-mVenus1-155-GGGS-TDP43 M337VAllows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays
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162613pCS2plus-mVenus1-155-I152L-GGGSAllows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays
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162614pCS2plus-mVenus1-155-I152L-GGGS-TDP-43Allows for transcription of improved mVenus I152L fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays
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162615pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337VAllows for transcription of improved mVenus I152L fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays
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162616pCS2plus-mCerulean 156-239-GGGSAllows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays
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162617pCS2plus-mCerulean 156-239-GGGS-TDP-43Allows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays
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162618pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337VAllows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays
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162624pCS2plus-mKate2Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays
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Recombinant Antibodies from Article