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We narrowed to 5,523 results for: golden gate

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Showing: 2141 - 2160 of 5523 results

  10. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

    Plasmid
    #182356
    Purpose
    Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
    Depositor
    Christian Froekjaer-Jensen
    Publication
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Expression
    Worm
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182356-plasmid-map-sequence-id-361657

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  16. pKMW123_SpRY-Cas9_U6-entry

    Plasmid
    #244820
    Purpose
    Mammalian expression of SpRY Cas9 with Golden Gate-compatible sgRNA spacer cassette
    Depositor
    Jennifer Doudna
    Publication
    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpRY Cas9
    Use
    CRISPR
    Tags
    3xFLAG-SV40 NLS and Nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…
    Promoter
    CAG, U6
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244820-plasmid-map-sequence-id-486582

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  18. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  19. pPD1178-LPDV promoterless PuroR-P2A-miRFP720

    Plasmid
    #201537
    Purpose
    Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene
    Depositor
    Joshua Leonard
    Publication
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Insert
    Promoterless PuroR-P2A-miRFP720
    Expression
    Mammalian
    Promoter
    None
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201537-plasmid-map-sequence-id-401330

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Showing: 2141 - 2160 of 5523 results