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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,282 results for: golden gate

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Showing: 2141 - 2160 of 5282 results

  3. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-487610

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  8. pEHE51

    Plasmid
    #250522
    Purpose
    Vector for sgRNA expression in Acinetobacter baumannii (CRISPRi). Modified version of pYDE007 (Plasmid #194152) made Golden Gate compatible (BsaI) for sgRNA oligo insertion.
    Depositor
    Ralph Isberg
    Publication
    Hamami et al bioRxiv [Preprint]. 2025 Aug 4:2024.01.0
    Insert
    Two BsaI sites (T-BsaI-BfuI-BsaI-A) inserted between the J23119 promoter and dCas9 handle/gRNA scaffold
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    250522-plasmid-map-sequence-id-497877

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Showing: 2141 - 2160 of 5282 results