We narrowed to 3,637 results for: golden gate

1. pEPOR1CB0081

   Plasmid

   #117561

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtMPK19 {AT3G14720} in A. thaliana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_AtMPK19 {AT3G14720} A. thaliana
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 (pICSL90002)

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPOR1CB0114

   Plasmid

   #117553

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   At U6-26 (pICSL90002)

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPOR1CB0029

   Plasmid

   #117541

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   crRNA (LbCas12a)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPOR1CB0028

   Plasmid

   #117540

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   crRNA (AsCas12a)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPOR1CB0022

   Plasmid

   #117537

   Purpose

   Level1 Golden Gate Cassette: estended stem sgRNA cassette for SpCas9 targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   sgRNA (Sp Cas9) - extended stem
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEPOR0SP0012

   Plasmid

   #117533

   Purpose

   Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   eSaCas9 (no stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   R499A,Q500K,R654A,G655R

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPOR0SP0018

   Plasmid

   #117528

   Purpose

   Level0 Golden Gate part: CDS, Sp-eCas9 1.1

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-eCas9 1.1 (with stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K848A, K1003A, R1060A

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR0SP0015

   Plasmid

   #117525

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-KA (no stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K855A

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPOR0SP0014

   Plasmid

   #117524

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-KA

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-KA (with stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K855A

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEPOR0SP0001

    Plasmid

    #117523

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-DE (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D1135E

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPOR0SP0009

    Plasmid

    #117522

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL90005

    Plasmid

    #117520

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-h (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPOR0SP0008

    Plasmid

    #117535

    Purpose

    Level0 Golden Gate part: CDS, AsCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    AsCas12a (with stop codon)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGGF004

    Plasmid

    #48845

    Purpose

    Provides a D-alanine resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    p35S::D-AlanineR::t35S
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    internal BsaI recognition site in 35S promoter re…

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEE082 FoMV

    Plasmid

    #234811

    Purpose

    To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-BbsI-PITCh

    Plasmid

    #127875

    Purpose

    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJPC596 FoMV-AmCyan

    Plasmid

    #234812

    Purpose

    To express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

    Plasmid

    #139236

    Purpose

    mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tad-CBE(CCG3)

    Plasmid

    #235074

    Purpose

    Expresses Tad-CBE(CCG3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(CCG3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH-chl-roGFP2-synORP1

    Plasmid

    #191701

    Purpose

    Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-roGFP2-synORP1
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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