1. Myr-(iSH2-p85)-p110alpha-Myc

   Plasmid

   #1410

   Depositor

   Lewis Cantley

   Insert

   PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pBXC3H

   Plasmid

   #47068

   Purpose

   FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p7XC3GH

   Plasmid

   #47066

   Purpose

   FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBXNH3

   Plasmid

   #47067

   Purpose

   FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FASN

   Plasmid

   #38793

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   FASN (FASN Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. FASN

   Plasmid

   #38794

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   FASN (FASN Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pTLNXA7

   Plasmid

   #49033

   Purpose

   FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Xenopus oocytes expression

   Tags

   chicken alpha7 signal sequence

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBXCA3H

   Plasmid

   #47072

   Purpose

   FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), Avi-tag, and…

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYEXNH3

   Plasmid

   #49024

   Purpose

   FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBXC3GH

   Plasmid

   #47070

   Purpose

   FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pICXNH3

    Plasmid

    #49020

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYEXC3GH

    Plasmid

    #49027

    Purpose

    FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICXC3H

    Plasmid

    #49022

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYEXC3H

    Plasmid

    #49026

    Purpose

    FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYEXNHG3

    Plasmid

    #49025

    Purpose

    FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p142

    Plasmid

    #8385

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    LOX I/LOX II
    

    Use

    Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

16. p138

    Plasmid

    #8384

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    LOX I
    

    Use

    Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pBXNH3CA

    Plasmid

    #47069

    Purpose

    FX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), AVI-tag, and…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pREXC3GH

    Plasmid

    #47077

    Purpose

    FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Lactococcus lactis expression

    Tags

    3C protease site, GFP, and His

    Expression

    Mutation

    Promoter

    NIS (nisin)

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid

    #82327

    Purpose

    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)

    Depositor

    Elizabeth Stoll

    Insert

    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDXC3GMS

    Plasmid

    #49031

    Purpose

    FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), EGFP, MYC, a…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge