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pMSCV-IRES-GFP-3xFLAG-MyD88-V5

Plasmid

#131351

Purpose

Expresses MyD88 tagged with a 3xFLAG tag at its N-terminus and a V5 tag at its C-terminus

Depositor

Jonathan Kagan

Publication

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88 (Myd88 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9

Plasmid

#31736

Depositor

Ron Vale

Publication

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab9 (Rab9 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
Dll3ICD-HPC4 pBacPAK8-3

Plasmid

#17334

Depositor

Joan Conaway, Ronald Conaway

Publication

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Delta-like 3 (Dll3 Mouse)

Tags

HPC4

Expression

Insect

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pFM111

Plasmid

#40157

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-1N:FLAG (par-1 Nematode)

Tags

Flag and GST

Expression

Bacterial

Mutation

Contains aa 1-964

Promoter

tac

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pCM1.60

Plasmid

#19594

Depositor

Geraldine Seydoux

Insert

daz-1 prom in PDONRP4P1R

Expression

Worm

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-sCAG-GluClβ.CreON

Plasmid

#196996

Purpose

Cre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.

Depositor

David Bennett

Publication

Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0

Insert

GluClβ -YFP

Use

AAV and Cre/Lox

Tags

YFP

Promoter

short CAG

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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KG#371

Plasmid

#110882

Purpose

Expresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neurons

Depositor

Kenneth Miller

Publication

Edwards et al Genetics. 2013 May;194(1):143-61. doi: 1

Inserts

unc-129 promoter
CTNS-1a-mCherry cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region

Tags

intron-mCherry

Expression

Bacterial

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry-pBirA-HA

Plasmid

#154458

Purpose

expresses mCherry-pBirA-HA/biochemical assay

Depositor

Hyun-Woo Rhee

Publication

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry-pBirA-HA

Expression

Mammalian

Promoter

CMV-F, T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pUF-AsCpf1-pre-gRNA

Plasmid

#137849

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Publication

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

AsCpf1 and pre-gRNA cassette

Use

CRISPR

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM44

Plasmid

#133653

Purpose

Integration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

Depositor

Zhiping Xie

Publication

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-SNF7-mCherry (SNF7 Budding Yeast)

Tags

mCherry

Expression

Yeast

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Bgh-GFP-Farpl

Plasmid

#115501

Purpose

expression of rat Farp1

Depositor

Thomas Biederer

Publication

Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

Insert

Farp1 (Farp1 Rat)

Tags

GFP

Expression

Mammalian

Promoter

chicken beta-actin

Available since

Dec. 26, 2018

Availability

Academic Institutions and Nonprofits only

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Ad-EphA3 WT

Plasmid

#102725

Purpose

Expression of WT EphA3

Depositor

Jin Chen

Publication

Frieden et al Dev Dyn. 2010 Dec;239(12):3226-34. doi:

Insert

EphA3 (Epha3 Mouse)

Use

Adenoviral

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSF11-wmCardinal

Plasmid

#197251

Purpose

Expresses the protein of wmCardinal in neurons of C. elegans

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmCardinal

Expression

Worm

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCM3.11

Plasmid

#19605

Depositor

Geraldine Seydoux

Insert

pgl-1 ORF+3' in pDONR201 (pgl-1 Nematode)

Expression

Worm

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pLJM1-TMEM192-FLAG

Plasmid

#164977

Purpose

Lentiviral vector for TMEM192 expression in mammalian cells

Depositor

Roberto Zoncu

Publication

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUF-LbCpf1-pre-gRNA

Plasmid

#137848

Purpose

LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Publication

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

LbCpf1-NLS-FLAG and gRNA cassette

Use

CRISPR

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112922

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

Depositor

Kosuke Yusa

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Gateway Cloning

Expression

Mammalian

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Ngn3ER-PuroR-mCherry

Plasmid

#197385

Purpose

To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

Depositor

Qiao Zhou

Publication

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

Ngn3ER-2A-PuroR-2A-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV_SEPT2-msfGFP

Plasmid

#180318

Purpose

mammalian expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

Plasmid

#135812

Purpose

This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

Depositor

Nadia Mercader Huber

Publication

Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

Insert

TagRFP

Use

Zebrafish

Promoter

UAS

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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