We narrowed to 5,091 results for: codon optimized

1. pIntegrase_9

   Plasmid

   #60580

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-APOBEC1-YTHmut

   Plasmid

   #200159

   Purpose

   For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   APOBEC1-YTHmut
   

   Use

   Tags

   6xHis, HA, and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   LacZ

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAS9-TPC

   Plasmid

   #61478

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PcUbi4-2

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBLO1811_arC9_noNLS_human

   Plasmid

   #74494

   Purpose

   Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   arC9
   

   Use

   CRISPR

   Tags

   t2a mCherry (C-terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBY1_FnCpf1cg

   Plasmid

   #104622

   Purpose

   CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

   Depositor

   Sheng Yang

   Article

   Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   codon-optimized C.glutamicum

   Promoter

   hsp60

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPP394

   Plasmid

   #158800

   Purpose

   Plasmid containing Homo sapiens codon optimized CasΦ-1 and a SapI-GG stuffer spacer for editing in human cells.

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUDP293

   Plasmid

   #204228

   Purpose

   Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

   Depositor

   Jean-Marc Daran

   Article

   Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

   Inserts

   Eubacterium rectale cas12a (Ercas12a)
   BsaI_GFP drop-out_BsaI
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mutation

   codon optimized for S. cerevisiae

   Promoter

   Saccharomyces cerevisiae PGK1 and Saccharomyces c…

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ALIX-His(1-868, P801G)

   Plasmid

   #186808

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   ALIX-His (1-868, P801G) (PDCD6IP Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Proline 801 to Glycine

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCMV_SLEV_PrME

   Plasmid

   #207309

   Purpose

   Mammalian expression of St. Louis encephalitis virus PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   SLEV_PrME (POLY St. Louis encephalitis virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+_NLS-Tir1-HA

    Plasmid

    #117716

    Purpose

    mRNA synthesis of NLS-TIR1-HA for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized TIR1 for murine expression

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTU424

    Plasmid

    #178983

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYZ959

    Plasmid

    #192603

    Purpose

    TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    ScTEF1

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET32-sLatherin

    Plasmid

    #61685

    Purpose

    bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

    Depositor

    Brian Smith

    Article

    Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

    Insert

    Latherin (LATH horse)
    

    Use

    Tags

    HIS and Trx-Tag thioredoxin protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

14. His-Tev-WWP2

    Plasmid

    #186807

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-WWP2 (WWP2 Human)
    

    Use

    Tags

    His6-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR - AAVS1 sgRNA

    Plasmid

    #70661

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against AAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pBLO1811_Cas9_noNLS_human

    Plasmid

    #74490

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBY3_ligD

    Plasmid

    #104624

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACAGW-oChiEFtdTomato-AAV

    Plasmid

    #32846

    Purpose

    AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

    Depositor

    Rory McQuiston

    Article

    Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

    Insert

    oChiEF
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    CAGG

    Available since

    March 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAP24

    Plasmid

    #70190

    Purpose

    Modular vector for secreted luciferase reporter expression

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

    Insert

    sLucopt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENO1-iRFP-NATr

    Plasmid

    #129731

    Purpose

    Integration plasmid to overexpress iRFP in Candida albicans

    Depositor

    Robert Wheeler

    Article

    Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

    Insert

    iRFP-Candida albicans codon-optimized
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO1 from Candida albicans

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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