We narrowed to 3,795 results for: golden gate

1. pICH-chl-roGFP2-ORP1

   Plasmid

   #191699

   Purpose

   Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   chl-roGFP2-ORP1
   

   Use

   Binary agrobacterium ti plasmid

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBTK151

   Plasmid

   #183127

   Purpose

   BTK Type 4 reverse promoter part for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al Science. 2020 Jan 31;367(6477):573-576.

   Insert

   Reverse CP25 promoter and forward rpoC terminator for dsRNA expression
   

   Use

   Synthetic Biology

   Available Since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pPD535 VP16-ZF129

   Plasmid

   #138768

   Purpose

   Constitutive expression of VP16-ZF129 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF129
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPD303 VP64-ZF43

   Plasmid

   #138835

   Purpose

   Constitutive expression of VP64-ZF43 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP64-ZF43
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

   Plasmid

   #162804

   Purpose

   STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-BsmBI_IRES_Blasticidin
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPD158 dsRedExpress2-ZFi37

   Plasmid

   #138797

   Purpose

   Constitutive expression of dsRedExpress2-ZFi37 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   dsRedExpress2-ZFi37
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZA31

   Plasmid

   #158509

   Purpose

   In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-4xMyc. Km plant selection marker.

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1-4xMyc
   

   Tags

   4xMyc

   Expression

   Plant

   Mutation

   L17E

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD385 VPR-ZF43(RAAA)

   Plasmid

   #138824

   Purpose

   Constitutive expression of VPR-ZF43(RAAA) under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VPR-ZF43(RAAA)
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   R15A, R43A, R71A

   Promoter

   CMV

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBTK150

   Plasmid

   #183126

   Purpose

   BTK Type 2 promoter part for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al Science. 2020 Jan 31;367(6477):573-576.

   Insert

   Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression
   

   Use

   Synthetic Biology

   Available Since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pIIS18-SapI

    Plasmid

    #128599

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIIS18-BsmBI

    Plasmid

    #128648

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ138A

    Plasmid

    #69280

    Purpose

    Golden Gate entry vector; 8th gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ137A

    Plasmid

    #69279

    Purpose

    Golden Gate entry vector;7th gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPD392 VPR-ZF92

    Plasmid

    #138841

    Purpose

    Constitutive expression of VPR-ZF92 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF92
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

    Plasmid

    #139230

    Purpose

    mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPD107 VP16-ZF43(RARR)

    Plasmid

    #138812

    Purpose

    Constitutive expression of VP16-ZF43(RARR) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43(RARR)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R15A

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD530 VP16-ZF42

    Plasmid

    #138760

    Purpose

    Constitutive expression of VP16-ZF42 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF42
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPD531 VP16-ZF54

    Plasmid

    #138762

    Purpose

    Constitutive expression of VP16-ZF54 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF54
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPD527 VP16-ZF14

    Plasmid

    #138756

    Purpose

    Constitutive expression of VP16-ZF14 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF14
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNM3746

    Plasmid

    #154109

    Purpose

    loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::His-58::FRT3
    mex-5p::FLP D5::sl2::mNG::glh2 3'
    

    Use

    Cre/Lox; Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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