We narrowed to 3,637 results for: golden gate

1. p0AmpBC-natR

   Plasmid

   #237270

   Purpose

   Carries the codon-optimized X. dendrorhous natR gene

   Depositor

   Eric Young

   Article

   Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

   Insert

   natR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p0AmpBC-Nluc

   Plasmid

   #237271

   Purpose

   Carries the codon-optimized X. dendrorhous NLuc gene

   Depositor

   Eric Young

   Article

   Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

   Insert

   Nluc
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SMT503 - S77A

   Plasmid

   #226842

   Purpose

   Plasmid that encodes a B. Subtilis lipase (CLIP-BsLipA-eGFP) gene fusion with the S77A catalytic mutant. Compatible with PURExpress for in vitro transcription/translation. Chloramp

   Depositor

   Polly Fordyce

   Article

   Thompson et al Adv Mater Interfaces 2024, 12 (3), 24004

   Insert

   Lipase A
   

   Use

   Synthetic Biology

   Tags

   SNAP and eGFP

   Expression

   Bacterial

   Mutation

   R33Q, D34N, K35D, S77A, K112D, M134D, Y139C, I157M

   Promoter

   T7

   Available since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SpRY-Tad-CBE(CCG3)

   Plasmid

   #235083

   Purpose

   Expresses SpRY-Tad-CBE(CCG3) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCG3)-SpRY Cas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Tad-CBE(CCA2)

   Plasmid

   #235069

   Purpose

   Expresses Tad-CBE(CCA2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCA2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Tad-CBE(CCC2)

   Plasmid

   #235072

   Purpose

   Expresses Tad-CBE(CCC2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCC2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Tad-CBE(GCA1)

   Plasmid

   #235075

   Purpose

   Expresses Tad-CBE(GCA1) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(GCA1)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tad-CBE(GCT1)

   Plasmid

   #235076

   Purpose

   Expresses Tad-CBE(GCT1) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(GCT1)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Tad-CBE(GCT2)

   Plasmid

   #235077

   Purpose

   Expresses Tad-CBE(GCT2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(GCT2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tad-CBE(GCG1)

    Plasmid

    #235079

    Purpose

    Expresses Tad-CBE(GCG1) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(GCG1)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SpRY-Tad-CBE(ACC2)

    Plasmid

    #235081

    Purpose

    Expresses SpRY-Tad-CBE(ACC2) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACC2)-SpRY Cas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SpRY-Tad-CBE(ACC3)

    Plasmid

    #235082

    Purpose

    Expresses SpRY-Tad-CBE(ACC3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACC3)-SpRY Cas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Tad-CBE(ACC3)

    Plasmid

    #235062

    Purpose

    Expresses Tad-CBE(ACC3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACC3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Tad-CBE(ACG1)

    Plasmid

    #235063

    Purpose

    Expresses Tad-CBE(ACG1) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACG1)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Tad-CBE(TCC1)

    Plasmid

    #235066

    Purpose

    Expresses Tad-CBE(TCC1) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(TCC1)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSIG1

    Plasmid

    #226908

    Purpose

    MoClo-compatible vectors for dsRNA production in E. coli

    Depositor

    Li Hung Chen

    Article

    Wu et al Plant Methods. 2025 Jul 11;21(1):96. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSIG2

    Plasmid

    #226909

    Purpose

    MoClo-compatible vectors for dsRNA production in E. coli

    Depositor

    Li Hung Chen

    Article

    Wu et al Plant Methods. 2025 Jul 11;21(1):96. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKG1334_pHarbor-PiggyBac_backbone

    Plasmid

    #239705

    Purpose

    PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated integration

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKG0586_pPV2-mRuby2

    Plasmid

    #239695

    Purpose

    BsaI Golden Gate assembly plasmid encoding the mRuby2 CDS

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKG2039_pPV1-CAG

    Plasmid

    #239686

    Purpose

    BsaI Golden Gate assembly plasmid encoding the CAG promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    CAG promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge