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pICSL1P6_AtU626Ter26

Plasmid

#191774

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-MiniE-mEm

Plasmid

#181991

Purpose

MiniE genetically encoded tag on H2B-mEmerald to be used with MiniR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

H2B-MiniE-mEm

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#3

Plasmid

#174325

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#2

Plasmid

#174324

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET-11a HIV-1 P6

Plasmid

#154201

Purpose

Bacterial expression of Gag P6 from HIV-1 HXB2 strain

Depositor

Lalit Deshmukh

Article

Ramaraju et al Biochemistry. 2021 Aug 3. doi: 10.1021/a

Insert

Gag P6 protein from HIV-1 clone HXB2

Tags

GB1-6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K

Plasmid

#112652

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K

Expression

Yeast

Mutation

R59K (GAP-dead)

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K

Plasmid

#112647

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K

Expression

Yeast

Mutation

R54K

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL7007

Plasmid

#136946

Purpose

Malachite green aptamer with stability hairpin, bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663K

Plasmid

#92239

Purpose

Express R663K mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663K mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Lysine

Promoter

T7 promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663Q

Plasmid

#92240

Purpose

Express R663Q mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663Q mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Glutamine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-H698A

Plasmid

#92237

Purpose

Express H698A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663A

Plasmid

#92238

Purpose

Express R663A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJK040 (pET-PurQ-A128T)

Plasmid

#73840

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Expression

Bacterial

Mutation

A128T mutant

Promoter

T7

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJK044 (pET-PurQ-wt)

Plasmid

#73839

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJK324

Plasmid

#71838

Purpose

Cloned region contains Acetobacter aceti 1023 purE gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK340

Plasmid

#71837

Purpose

Cloned region contains Acetobacter aceti 1023 purE(H59N) gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK346

Plasmid

#71836

Purpose

Cloned region contains Acetobacter aceti 1023 purE(H59D) gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to aspartate

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_PA-GFP

Plasmid

#62412

Purpose

MXS_chaining vector with PA-GFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PA-GFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_loxP

Plasmid

#62430

Purpose

MXS_chaining vector with loxP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

loxP site

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_linker

Plasmid

#62431

Purpose

MXS_chaining vector with linker

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Linker 12AA Gly-/Ser-rich

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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