We narrowed to 9,409 results for: try

1. pENTR-R4R3-2xmRuby-N

   Plasmid

   #113753

   Purpose

   Gateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mRuby2-mRuby2
   

   Use

   CRISPR; Gateway entry vector

   Tags

   2xmRuby2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-R4R3-2xmRuby-C

   Plasmid

   #113756

   Purpose

   Gateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mRuby2-mRuby2
   

   Use

   CRISPR; Gateway entry vector

   Tags

   2xmRuby2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. RNAPII_CTD27-52_S1966C

   Plasmid

   #98681

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966C

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S1966C

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSUPER-retro-puro-shNup88-XbaI

   Plasmid

   #87328

   Purpose

   To express shRNA against human Nup88

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   shRNA against human Nup88
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a - MPX

   Plasmid

   #89619

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Mitocondrial Tryparedoxin peroxidase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-CMV.Pro

   Plasmid

   #79486

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   CMV Promoter
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUPER-retro-puro-shNup93-XhoI

   Plasmid

   #87330

   Purpose

   To express shRNA against human Nup93

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   shRNA against human Nup93
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30-roGFP1-iR

   Plasmid

   #83314

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

   Insert

   roGFP1-iR
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

   Promoter

   T5

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pFOSGE_DUM1B01

   Plasmid

   #71089

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Clk (Clk Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pJK278

    Plasmid

    #71134

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to asparagine; additional N-…

    Promoter

    T7

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK130

    Plasmid

    #71129

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changed valine-319 to phenylalanine

    Promoter

    T7

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK173

    Plasmid

    #71831

    Purpose

    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genes

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFOSGE_COF2C10

    Plasmid

    #70927

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    okr (okr Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pJK320

    Plasmid

    #71144

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to serine; additional N-term…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK319

    Plasmid

    #71143

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to alanine; additional N-ter…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK318

    Plasmid

    #71142

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to glutamine; additional N-t…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK315

    Plasmid

    #71141

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to glycine; additional N-ter…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK314

    Plasmid

    #71140

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to valine; additional N-term…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK174

    Plasmid

    #71835

    Purpose

    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7 and endogenous

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK279

    Plasmid

    #71135

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to aspartate; additional N-t…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge