We narrowed to 5,135 results for: codon optimized

1. PARP1 FL

   Plasmid

   #169815

   Purpose

   Expresses codon optimised full length His tagged PARP1 in bacterial cells

   Depositor

   David Neuhaus

   Article

   Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

   Insert

   Poly[ADP-ribose] polymerase 1 (PARP1 Human, Synthetic, Codon optimised)
   

   Tags

   MKHHHHHHMKQ

   Expression

   Bacterial

   Mutation

   Valine 762 mutated to an alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. His-ZZ-TEV-SNAPf-DYNC2H1

   Plasmid

   #132524

   Purpose

   Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNC2H1 (DYNC2H1 Human)
   

   Tags

   8x His, Linker-TEV-linker-TEV, Precission cleavag…

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pD603 H77 E1E2

   Plasmid

   #86983

   Purpose

   Mammalian expression vector encoding codon optimised Heptitis C virus H77 glycoproteins E1E2

   Depositor

   Joe Grove

   Insert

   HCV E1E2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ152

   Plasmid

   #69303

   Purpose

   Gateway entry vector with pco-dCas9-VP64

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-VP64 (Plant codon-optimized)
   

   Use

   CRISPR

   Mutation

   VP64 activator fusion; NLS fusion

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Twin-Strep(R)-Tev-GSGS-Ubiquitin

   Plasmid

   #186803

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   Twin-Strep(R)-Tev-GSGS-Ubiquitin
   

   Tags

   GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

   Expression

   Bacterial

   Mutation

   Strep tag Lysine's mutated to Arginine

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRBC-sfGFP-134/150TAG

   Plasmid

   #174077

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   D134TAG + N150TAG

   Promoter

   T7

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR-Cas3

   Plasmid

   #209427

   Purpose

   Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

   Depositor

   Tilmann Weber

   Article

   Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

   Insert

   codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PtipA; crRNA under control of PermE*

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-NLS-HA-Bxb1

   Plasmid

   #51271

   Purpose

   Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS-HA-Bxb1
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

    Plasmid

    #104394

    Purpose

    Yeast MCP-GFP expression

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    MCP-NLS-2xyeGFP
    

    Expression

    Bacterial and Yeast

    Mutation

    MCP fused to NLS(SV40) and 2x yeast codon optimiz…

    Promoter

    CYC1

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIW601-KmCRISPR

    Plasmid

    #98907

    Purpose

    K. marxianus CRISPR Plasmid for sgRNA cloning

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Alpha-Synuclein-His

    Plasmid

    #186802

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    Alpha-Synuclein-His (SNCA Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. iSpyMac

    Plasmid

    #155014

    Purpose

    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

    Insert

    iSpyMac
    

    Expression

    Mammalian

    Mutation

    PID grafted onto SpyCas9 N-terminal domain, R221K…

    Promoter

    EF1ɑ

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBudORF1-CH

    Plasmid

    #51290

    Purpose

    expresses the codon optimized ORF1 of L1RP with his tag

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1 ORF1 (L1RE1 Human)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TurboID-mNeongreen-3xFLAG fusion pos3

    Plasmid

    #172864

    Purpose

    C. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein

    Depositor

    Mario de Bono

    Article

    Artan et al J Biol Chem. 2021 Sep;297(3):101094. doi

    Insert

    TurboID
    

    Use

    Unspecified

    Tags

    FLAG, mNeongreen, and myc

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti6.3-sRGN3.1-BlastR

    Plasmid

    #220964

    Purpose

    Expresses human codon-optimized sRGN3.1

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    sRGN3.1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Dsup pLVX-Puro

    Plasmid

    #180635

    Purpose

    Expresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio tolerance

    Depositor

    Christopher Mason

    Article

    Westover et al bioRxiv 2020.11.10.373571

    Insert

    Dsup
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EcSTH-LbNOX

    Plasmid

    #211924

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

    Depositor

    Binghui Li

    Article

    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

    Insert

    EcSTH-LbNOX
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCgCas9_recT

    Plasmid

    #153337

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5FRT/TO_HA-mAID-nanobody

    Plasmid

    #117713

    Purpose

    Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mAID-nanobody
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge